Measure UV-Vis
Thermo Scientific NanoDrop One User Guide 149
Best practices for UV-Vis measurements
• Ensure the sample absorbance is within the instrument’s absorbance detection limits.
• Blank with the same buffer solution used to resuspend the analyte of interest. The
blanking solution should be a similar pH and ionic strength as the analyte solution.
•Run a blanking cycle to assess the absorbance contribution of your buffer solution. If the
buffer exhibits strong absorbance at or near an analysis wavelength, you may need to
choose a different buffer or application. See Choosing and Measuring a Blank for more
information.
• For micro-volume measurements:
– Ensure pedestal surfaces are properly cleaned and conditioned.
– Ensure samples are homogeneous before taking a measurement. Avoid introducing
bubbles when mixing and pipetting.
– Follow best practices for micro-volume measurements.
– Use a 1-2 μL sample volume. See Recommended Sample Volumes for more
information.
• For cuvette measurements (NanoDrop One
C
instruments only), use compatible cuvettes
and follow best practices for cuvette measurements.
Related Topics
• Measure a Micro-Volume Sample
• Measure a Sample Using a Cuvette
• Best Practices for Micro-Volume Measurements
• Best Practices for Cuvette Measurements
• Prepare Samples and Blanks
• Basic Instrument Operations
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