Measure UV-Vis
152 NanoDrop One User Guide Thermo Scientific
Settings for UV-Vis Measurements
To show the UV-Vis settings, from the UV-Vis measurement screen, tap > UV-Vis
Setup.
Related Topics
• Instrument Settings
Setting Available Options Description
Monitored
wavelengths
Enter up to 40
wavelengths between
190 nm and 850 nm
User-defined wavelengths to be measured and reported at run
time. Absorbance values for the first three entered wavelengths
are displayed in the measurement screen. To see absorbance
values for 8 monitored wavelengths, swipe left in the
measurement screen to show the Data table. To see all monitored
wavelengths, press and hold a sample row to show the Sample
Details screen (scroll up to display absorbance values for any
additional user-defined wavelengths).
Note: If Baseline Correction is selected, all displayed absorbance
values are the corrected values.
Automated Pathlength On or Off
(affects pedestal
measurements only)
Optional automated pathlength selection. Allows the software
to use the optimal (shorter) pedestal pathlength for high
concentration samples to help prevent detector saturation (see
Detection Limits for details).
• When selected, the shorter pathlength is used when any
wavelength between 220 nm and 850 nm has 10 mm
equivalent absorbance value of 12.5 or higher. For
wavelengths between 190 nm and 219 nm the change to the
shorter pathlength occurs when any wavelength in this range
has a 10 mm equivalent absorbance value of 10 or higher.
• When deselected, the pedestal pathlength is restricted to
10 mm across all wavelengths.
Note: In either case, displayed absorbance values have been
normalized to a 10 mm pathlength equivalent.
Baseline Correction On or off
Enter baseline correction
wavelength in nm or use
default value (750 nm)
Optional user-defined baseline correction. Can be used to
correct for any offset caused by light scattering particulates by
subtracting measured absorbance at specified baseline correction
wavelength from absorbance values at all wavelengths in sample
spectrum. As a result, absorbance of sample spectrum is zero at
specified baseline correction wavelength.
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