Measure Kinetics
Thermo Scientific NanoDrop One User Guide 167
Time Unit Select the unit for time-based measurements (seconds or
minutes).
Stage 1, 2, etc. Specify the available settings for each stage:
• Delay. Specify a delay before a stage starts.
• Interval Time. Specify the length of time between
measurements taken during this stage (minimum is
2 seconds). The first measurement occurs when the stage
starts (or after the delay is completed if a Delay is
specified).
Note: If two or more stages are specified with Delay set to
zero, two measurements occur at the same time (the
measurement at the beginning of the new stage directly
overlaps the one at the end of the previous stage).
• #Intervals. Specify the number of absorbance
measurements to take in this stage.
Note: Since the first measurement is taken when the stage
starts, the number of measurements reported for each
stage will be the #Intervals setting plus 1.
• Duration. Readout shows the total time required for this
stage, including any delay and all specified intervals.
The colored rows at the right (see image below) show the start
and end times for each stage; the colored boxes at the right
correspond with the specified delay and number of intervals
for each stage.
If no delay is specified, absorbance measurements are taken at
the start and end of each stage and after each specified
interval. If a delay is specified, as in stage 3 above, the first
measurement occurs at the start of the first interval. If the unit
is seconds in the example above, a total of 11 measurements
are taken at the following times over a period of 32 seconds:
Tab Setting Description
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