Thermo NanoDrop One - Micro-Volume Sampling-How it Works

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Learning Center
Micro-Volume Sampling—How it Works
176 NanoDrop One User Guide Thermo Scientific
Micro-Volume Sampling—How it Works
Surface Tension
Absorbance Spectrum
Sample Absorbance
Sample Concentration
Baseline Correction
Surface Tension
The NanoDrop One spectrophotometer uses surface tension to hold
a small volume of sample between two pedestals. The patented
sample retention system enables the measurement of highly
concentrated samples without the need for dilutions.
A fiber optic cable embedded in the upper pedestal leads to a xenon
light source. A second cable embedded in the lower pedestal leads to
a detector. When the instrument arm is down, the sample forms a
liquid column, essentially bridging the gap between the two fiber
optic cables.