Measure dsDNA, ssDNA or RNA
18 NanoDrop One User Guide Thermo Scientific
Related Topics
• Instrument Settings
Calculations for Nucleic Acid Measurements
Setting Available Options Description
Baseline Correction On or off
Enter baseline correction
wavelength in nm or use
default value (340 nm)
Optional user-defined baseline correction. Can be used to
correct for any offset caused by light scattering particulates by
subtracting measured absorbance at specified baseline correction
wavelength from absorbance values at all wavelengths in sample
spectrum. As a result, absorbance of sample spectrum is zero at
specified baseline correction wavelength.
The nucleic acid applications use the Beer-Lambert
equation to correlate absorbance with concentration.
Solving Beer’s law for concentration yields the equation
at the right.
Beer-Lambert Equation (solved for concentration)
c = A / ( * b)
where:
A = UV absorbance in absorbance units (AU)
= wavelength-dependent molar absorptivity coefficient (or extinction
coefficient) in liter/mol-cm
b = pathlength in cm
c = analyte concentration in moles/liter or molarity (M)
Note: Dividing the measured absorbance of a sample solution by its molar
extinction coefficient yields the molar concentration of the sample. See
Published Extinction Coefficients for more information regarding molar
vs. mass concentration values.
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