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Learning Center
NanoDrop One Viewer Software
Thermo Scientific NanoDrop One User Guide 269
Custom method settings
These settings are available for creating custom methods.
Setting Available Options
Measurement range Select spectral range in which method will acquire data.
Available options:
• Ultra-violet only (190 nm - 350 nm)
• Visible only (350 nm - 850 nm)
• Ultra-violet and visible (190 nm - 850 nm)
• Custom (specify starting and ending point in nanometers)
Notes:
•If a Baseline correction and/or Analysis wavelength correction are used, make sure
your selected spectral range includes your specified baseline correction and/or
analysis correction wavelength.
• For micro-volume absorbance measurements and measurements taken with
nonstandard (other than 10 mm) cuvettes, the spectra are normalized to a 10 mm
pathlength equivalent.
Analysis wavelength Monitor absorbance at specified wavelength (enter the wavelength in nanometers).
Note: The specified wavelength must fall within the selected measurement range.
The measurement results or the concentration will be calculated automatically using
the absorbance value at the specified wavelength and applying the selected method
type (factor or standard curve).
Result name Enter descriptive name for calculated concentration result (for example, “Nucleic
Acid”) and use adjacent drop down list to select appropriate unit. Result name appears
as column heading for reported concentration value.
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