Thermo NanoDrop One - Page 270

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Learning Center
NanoDrop One Viewer Software
270 NanoDrop One User Guide Thermo Scientific
Factor or Extinction
coefficient at 1 cm
pathlength (Formula
methods only)
Specify whether to use factor or extinction coefficient to calculate concentration
result:
• User-defined factor. Enter factor for 1 cm pathlength and use adjacent drop
down list to select appropriate unit. Equation below shows how factor is used to
calculate sample concentration:
c = (A * f) / b
where:
c = analyte concentration
A = absorbance in absorbance units (A)
f = factor (typically 1/
, where  = wavelength-dependent molar absorptivity
coefficient, or extinction coefficient)
b = pathlength in cm (determined at measurement time, then normalized to
10 mm (1 cm) pathlength equivalent)
• Extinction coefficient and molecular weight. Enter extinction coefficient for
1 cm pathlength and use adjacent drop down list to select appropriate unit.
Equation below shows how extinction coefficient is used to calculate sample
concentration:
c = A / ( * b)
where:
c = analyte concentration
A = absorbance in absorbance units (A)
 = wavelength-dependent molar absorptivity coefficient (or extinction
coefficient)
b = pathlength in cm (determined at measurement time, then normalized to
10 mm (1 cm) pathlength equivalent)
Setting Available Options