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Learning Center
NanoDrop One Viewer Software
Thermo Scientific NanoDrop One User Guide 271
Notes:
• Refer to product literature for information about factors and extinction
coefficients for specific materials.
• To set up a method that reports absorbance measurements only, select Factor or
Extinction Coefficient with the factor or extinction coefficient set to “1”.
• If specified unit for factor or extinction coefficient is based on mass (such as
mg/mL) and specified unit for calculated result is based on molarity (such as
pmol/μL) or vice versa, enter molecular weight and use adjacent drop down list
to select appropriate unit.
Standards (Standard curve
methods only)
Define the standards:
• Enter name and analyte concentration of each standard and a reference, if desired:
– Depending on the Curve Type setting, a standard curve can be generated
using two or more standards. (The software allows a reference and up to 7
standards.)
– All reference and standards solutions should be in the same buffer used to
resuspend the samples plus the same volume of reagent added to the samples.
– First standard can be a reference measurement. The reference solution
should contain none of the analyte of interest. (The reference measurement is
not the same as a blank measurement.)
– Concentration values for standards can be entered in any order but the
standards must be measured in the order in which they were entered;
however, best practice dictates that standards be measured from the lowest
concentration of the standard analyte stock to the highest.
– Concentration range of the standards must cover the dynamic range of the
assay and the expected range of the unknown samples. Sample analyte
concentrations are not extrapolated beyond the concentration of the highest
standard.
For more information, see Working with Standard Curves.
Setting Available Options
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