Thermo NanoDrop One - Reconditioning the Pedestals

4
Maintaining Your Instrument
Maintaining the Pedestals
Thermo Scientific NanoDrop One User Guide 285
Reconditioning the Pedestals
The pedestal surfaces may lose their “conditioned” properties over time, especially after
measurements with isopropyl alcohol or solutions that contain surfactants or detergents such
as the Bradford reagent. An unconditioned pedestal causes droplets on the lower pedestal to
“flatten out,” preventing proper formation of the liquid column when the arm is lowered. The
resulting spectrum may look “rough” or “jagged.”
If samples flatten out on the pedestal (rather than “beading up” or forming a rounded droplet)
or the liquid column breaks during a measurement, recondition the pedestals.
Supplies needed
• lint-free laboratory wipes
• PR-1 pedestal reconditioning kit (available from us or a local distributor)
• calibrated precision pipettor (0-2 μL)
• canned air
Related Topics
• Recondition Pedestals
• Clean Touchscreen
• Decontaminate Instrument
Unconditioned pedestal
(droplet flattens out)
Properly conditioned pedestal
(droplet beads up)