4
Maintaining Your Instrument
Instrument Diagnostics
Thermo Scientific NanoDrop One User Guide 295
2. Enter each lot-specific target absorbance value from the label on the PV-1 ampoule in its
associated entry box and then tap Done.
3. Lift the instrument arm and clean the upper and lower pedestal with a new laboratory
wipe.
4. Pipette 1 μL DI H
2
O onto the lower pedestal, lower the arm and tap Blank.
5. Lift the arm and clean both pedestals with a new wipe.
6. Pipette 1 μL PV-1 solution onto the lower pedestal and start the sample measurement:
•If Auto-Measure is On, lower arm
• If Auto-Measure is off, lower arm and tap Measure
After the measurement, the software displays the results. Here is an example of the
performance verification result screen.
7. Repeat step 6 to measure the PV-1 solution nine more times using a new 1 μL aliquot for
each measurement and cleaning both pedestals after each measurement.
Note Vigorously shake the ampoule of PV-1 solution, allow the liquid to collect in
the bottom portion of the ampoule and then follow standard practices to open it.
Swipe screen left to
view detailed results
Measured pathlengths
Drag tab down/up to
see more/less data
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