Thermo NanoDrop One - Calculations for Microarray Measurements

Measure Microarray
32 NanoDrop One User Guide Thermo Scientific
– edit any entries or settings
–tap Save Dye
Delete custom dye
– tap to select custom dye
–tap
Related Topics
• Instrument Settings
Calculations for Microarray Measurements
Tip Dyes pre-defined in the software cannot be deleted.
NOTICE Deleting a custom dye permanently removes the dye and all associated
information from the software.
As with the other nucleic acid applications, the
Microarray application uses a modification of the
Beer-Lambert equation to calculate sample
concentration where the extinction coefficient and
pathlength are combined and referred to as a “factor.”
The Microarray application offers six options (shown at
right) for selecting an appropriate factor for each
measured sample, to be used in conjunction with Beer’s
Law to calculate sample concentration.
If the factor is known, choose the Custom Factor option
and enter the factor in ng-cm/μL. Otherwise, choose the
option that best matches the sample solution.
Tip: Ideally, the factor or extinction coefficient should be
determined empirically using a solution of the study
nucleic acid at a known concentration using the same
buffer.
Available Options for Factors
• dsDNA (factor = 50 ng-cm/μL)
• ssDNA (factor = 33 ng-cm/μL)
• RNA (factor = 40 ng-cm/μL)
• Oligo DNA (calculated from user entered DNA nucleotide sequence)
• Oligo RNA (calculated from user entered RNA nucleotide sequence)
• Custom Factor (user entered factor between 15 ng-cm/μL and
150 ng-cm/μL
Note: See Sample Type for more information.