Thermo Scientific NanoDrop One User Guide 35
Measure using a Custom Factor
Measure Nucleic Acid using a Custom Factor
Use the Custom Factor application to quantify purified DNA or RNA samples that absorb at
260 nm with a user-defined extinction coefficient or factor. The application reports nucleic
acid concentration and two absorbance ratios (A260/A280 and A260/A230). A single-point
baseline correction can also be used.
To measure nucleic acid samples using a custom factor
Measures the concentration of
purified nucleic acids using a
custom factor for the calculations.
Measure using Custom Factor
Reported Results
Settings
Detection Limits
Calculations
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently
damage the quartz fiber optic cables.
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