Thermo Scientific NanoDrop One User Guide 53
Measure Protein A280
Measure Protein Concentration at A280
Use the Protein A280 application to quantify purified protein populations that contain amino
acids such as tryptophan or tyrosine, or cys-cys disulfide bonds, which exhibit absorbance at
280 nm. This application reports protein concentration measured at 280 nm and one
absorbance ratio (A260/A280). A single-point baseline correction can also be used. This
application does not require a standard curve.
To measure Protein A280 samples
Measures the concentration of
purified protein populations that
absorb at 280 nm.
Measure A280 Proteins
Reported Results
Settings
Detection Limits
Calculations
Note If your samples contain mainly peptide bonds and little or no amino acids, use the
Protein A205 application instead of Protein A280.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently
damage the quartz fiber optic cables.
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