Measure Protein A280
Thermo Scientific NanoDrop One User Guide 61
Protein editor
Use the Protein Editor to add a custom protein to the list of available protein sample types in
Protein A280 Setup and Proteins & Labels Setup.
To access the Protein Editor:
• from the Home screen, tap > Protein Editor
• from the Protein A280 or Proteins & Labels measurement screen, tap >
Settings > Protein Editor
Other protein
(
+ MW)
User entered molar
extinction coefficient
and molecular weight
Assumes protein has known molar extinction coefficient
() and molecular weight (MW), where:
(
molar
)*10=(
percent
)*(MW
protein
)
Enter MW in kiloDaltons (kDa) and molar extinction
coefficient (
) in M
-1
cm
-1
divided by 1000 (i.e.,
/1000). For example, for protein with molar extinction
coefficient of 210,000 M
-1
cm
-1
, enter 210.
Other protein
(
1%)
User entered mass
extinction coefficient
Assumes protein has known mass extinction coefficient
(). Enter mass extinction coefficient in L/gm-cm for
10 mg/mL (
1%) protein solution.
Baseline
Correction
On or off
Enter baseline correction
wavelength in nm or use
default value (340 nm)
N/A Corrects for any offset caused by light scattering
particulates by subtracting measured absorbance at
specified baseline correction wavelength from
absorbance values at all wavelengths in sample spectrum.
As a result, absorbance of sample spectrum is zero at
specified baseline correction wavelength.
Tip: If the sample has a modification that absorbs light
at 340 nm, select a different correction wavelength or
turn off Baseline Correction.
a
To add or edit a custom protein, use Protein Editor.
Setting Available Options
Mass Ext. Coefficient
(L/gm-cm)
Description
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