Measure Protein A280
64 NanoDrop One User Guide Thermo Scientific
Delete custom protein
– in Protein Editor, tap to select custom protein
–tap
Related Topics
• Instrument Settings
Detection Limits for Protein A280 Measurements
Detection limits and reproducibility specifications for purified BSA proteins are provided
here. The BSA lower detection limit and reproducibility values apply to any protein sample
type. The upper detection limits are dependent on the upper absorbance limit of the
instrument and the sample’s extinction coefficient.
To calculate upper detection limits for other (non-BSA) protein
sample types
To calculate upper detection limits in ng/μL for proteins, use the following equation:
(upper absorbance limit
instrumen
t
/mass extinction coefficient
sample
) * 10
For example, if the sample’s mass extinction coefficient at 280 nm is 6.67 for a 1%
(10 mg/mL) solution, the equation looks like this:
(550 / 6.67) * 10 = 824.6 (or ~825)
Related Topics
• Detection Limits for All Applications
Note Deleting a custom protein permanently removes the protein and all associated
information from the software.
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