Thermo Scientific NanoDrop One User Guide 69
Measure Proteins and Labels
Measure Labeled Protein Samples
Use the Proteins and Labels application to quantify proteins and fluorescent dyes for protein
array conjugates, as well as metalloproteins such as hemoglobin, using wavelength ratios. This
application reports protein concentration measured at 280 nm, an A269/A280 absorbance
ratio, and the concentrations and measured absorbance values of the dyes, allowing detection
of dye concentrations as low as 0.2 picomole per microliter. This information is useful for
evaluating protein/dye conjugation (degree of labeling) for use in downstream applications.
To measure labeled protein samples
Measures the concentration of
purified proteins that have been
labeled with up to two fluorescent
dyes.
Measure Labeled Proteins
Reported Results
Settings
Detection Limits
Calculations
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently
damage the quartz fiber optic cables.
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