Measure Proteins and Labels
76 NanoDrop One User Guide Thermo Scientific
Related Topics
• Instrument Settings
• Protein Editor
• Dye/Chromophore Editor
Detection Limits for Proteins and Labels Measurements
Detection limits and reproducibility specifications for purified BSA proteins and dyes that are
pre-defined in the software are provided here. The BSA lower detection limit and
reproducibility values apply to any protein sample type. The upper detection limits are
dependent on the upper absorbance limit of the instrument and the sample’s extinction
coefficient.
To calculate upper detection limits for other (non-BSA) protein
sample types
To calculate upper detection limits in ng/μL for proteins, use the following equation:
(upper absorbance limit
instrumen
t
/mass extinction coefficient
sample
) * 10
For example, if the sample’s mass extinction coefficient at 280 nm is 6.67 for a 1%
(10 mg/mL) solution, the equation looks like this:
(550 / 6.67) * 10 = 824.6 (or ~825)
Related Topics
• Detection Limits for All Applications
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