Thermo Scientific NanoDrop One User Guide 79
Measure Protein A205
Measure Protein Concentration at A205
Use the Protein A205 application to quantify purified peptides and other proteins that
contain peptide bonds, which exhibit absorbance at 205 nm. This application reports protein
concentration and two absorbance values (A205 and A280). A single-point baseline
correction can also be used. This application does not require a standard curve.
To measure Protein A205 samples
Measures the concentration of
purified protein populations that
absorb at 205 nm.
Measure A205 Proteins
Reported Results
Settings
Detection Limits
Calculations
Note If your samples contain mainly amino acids such as tryptophan or tyrosine, or
cys-cys disulfide bonds, use the Protein A280 application instead of Protein A205.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently
damage the quartz fiber optic cables.
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