Thermo Orbitrap Fusion Series - Before Operating the Orbitrap Fusion Series System; Checking the System Mode; Checking the Vacuum Pressure Levels

7
Daily Operation
Before Operating the Orbitrap Fusion Series System
62 Orbitrap Fusion Series Hardware Manual Thermo Scientific
Before Operating the Orbitrap Fusion Series System
Follow these preventive maintenance procedures every day before beginning the first analysis:
• Checking the System Mode
• Checking the Vacuum Pressure Levels
• Checking the Gas Supplies
Checking the System Mode
Make sure that the system is turned on. See “Turning On the Mass Spectrometer” on page 52.
Checking the Vacuum Pressure Levels
Before beginning your daily operation, check the vacuum pressure levels in the system and
check for major air leaks in the system. If there is a major air leak, the system does not pump
down to sufficient levels to turn on the system. In the Tune window, a green square, ,
indicates that the readback value is good.
 To check the vacuum pressures
Make sure that the Vacuum LED on the front of the mass spectrometer is green, which
indicates that the pressure gauges are within their threshold values.
 To check the system for major air leaks
Listen for a rush of air or a hissing sound coming from the mass spectrometer.
Possible causes of a major leak might be a loose or disconnected fitting, an improperly
positioned O-ring, or an open valve.
CAUTION For proper performance, operate the Orbitrap Fusion Series system at the
proper vacuum levels. Operating the system with poor vacuum levels can cause reduced
sensitivity and reduced electron multiplier life.
IMPORTANT
•See page 55 for the gauges’ threshold values. If the readback values are higher
than normal, there might be an air leak or the helium flow might be insufficient.
• If the UHV pressure is above 5 × 10
–8
Torr in the analyzer region and the system
restarted within the last 30 to 60 minutes, wait another 30 minutes and recheck
the pressure. If the pressure decreases with time, check the pressure periodically
until it is within the typical range for the mass spectrometer.
• If the pressure remains high, the system might have an air leak.