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Thermo Orion Star - Calibrating with Water Saturated Air

Thermo Orion Star
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VIII-7
Orion Star Series Meter
Thermo Electron Corporation
Dissolved Oxygen Technique
Calibrating with Water Saturated Air
The calibration function must be enabled in Setup before
the calibration is started.
1. Prepare the calibration sleeve
* by removing the reservoir
cap from the bottom of the sleeve. Remove the sponge
from the cap and saturate it with water. Squeeze all excess
water out of the sponge so that it is merely damp. Replace
the sponge into the cap and replace the cap onto the
calibration sleeve.
* A standard 300 mL BOD bottle may also be used for AIR calibration.
Fill the bottom of the bottle with 50 mL of distilled water. Be sure to
use the BOD funnel accessory (Orion Cat. Nos. 080360 or 080160,
depending on the probe in use). Place the probe into the funnel
so that it is just above the stirring paddle. Ensure that there are no
drops of water on the surface of the membrane. Ensure that the
water level is 1/2 inch below the tip of the probe. Allow at least
30 minutes for the bottle to equilibrate.
2. Place the dissolved oxygen probe into the calibration
sleeve. Ensure that the probe membrane does not come
into contact with the sponge in the calibration sleeve. Also
ensure that there are no drops of water on the membrane.
3. Make sure Setup has
calibration set.
4. To initiate the Air calibration, press
. The “cal” indicator
will light up.
5. While the electrode signal is stabilizing, the meter will
flash a dissolved oxygen reading (based upon the current
calibration data) in the main field. Once the input has
stabilized, 102.3 % saturation will be displayed for three
seconds. The meter will return to measure mode.

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