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WESCOR Vapro 5520 - User Manual

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Summary

Introduction

Users Manual Overview

Provides an overview of the Vapro User's Manual and its contents, emphasizing efficient operation and instrument care.

Customer Service

Contact information and procedures for obtaining technical assistance and support for the Vapro osmometer.

Vapro System Description

Details the Vapro osmometer's advanced electronic adaptation of the hygrometric method for vapor pressure determination.

How the Vapro Works

Explains the operational principle of the Vapro osmometer, including sample aspiration, vapor pressure equilibrium, and measurement cycle.

Instrument Front Panel Controls

Description of the Vapro osmometer's front panel controls including display, keypad, and indicators.

Instrument Top Features

Details the TC Head Access Cover on the instrument top for cleaning and maintenance.

Instrument Rear Panel Features

Details the Vapro osmometer's rear panel components like serial data port and power entry module.

Power Entry Module and Controls

Explains the power entry module, fuse door, power switch, and voltage selector on the Vapro osmometer.

Getting Started

Instrument Setup Sequence

Provides a step-by-step guide for the initial setup and operation of the Vapro osmometer.

Vapro Accessories and Supplies

Lists the accessories and supplies furnished with the Vapro osmometer and recommended cleaning procedures.

Setting Up the Osmometer

Step-by-step instructions for placing the osmometer, connecting the power cord, and preparing the sample chamber.

Micropipettor Information

Information on the micropipettor used with the Vapro osmometer, including positive displacement pipettors.

Using Optimol Osmolality Standards

Instructions on how to use Optimol ampoule osmolality standards for calibrating the Vapro osmometer.

Using Osmocoll II Serum Control

Guidance on using Osmocoll II serum control for Vapro osmometer calibration and quality assurance checks.

Changing Voltage Selector

Instructions on how to change the voltage selector on the Vapro osmometer to match the local power source.

Changing Fuses

Procedure for accessing and replacing fuses in the Vapro osmometer, including fuse specifications.

Operating the Vapro Osmometer

Operation Overview

Introduces the operation modes (Normal, Average) and functions of the Vapro osmometer.

Auto Repeat Mode

Details the Auto Repeat Mode for performing consecutive measurements and checking for contamination.

Process Delay Mode

Explains the Process Delay Mode for handling samples that may not readily evaporate or require longer equilibration.

Function Menu Options

Describes the Function Menu options: Calibrate, Clean Test, Delete Result, and Mode Menu.

Clean Test Procedure

Details the Clean Test procedure for checking thermocouple cleanliness and contamination levels.

Delete Result Function

Explains how to delete the last result from the data group using the Vapro osmometer.

Returning to Mode Menu

Instructions on how to exit the Function Menu and return to the Mode Menu.

Loading Samples

Provides detailed instructions on how to properly load samples into the Vapro osmometer's sample chamber.

Cleaning the Sample Holder

Step-by-step guide on how to remove and clean the sample holder for the Vapro osmometer.

Running the Clean Test

Instructions for running the Clean Test to assess thermocouple cleanliness and contamination levels.

Calibrating the Instrument

Explains the factors affecting calibration accuracy and provides guidance on instrument calibration procedures.

Standby or Waiting Periods

Details the instrument's behavior during standby or waiting periods and the importance of proper chamber management.

Serial Data Output

Information on the Vapro osmometer's serial data port, its configuration, and how to send data to an external device.

Preventive Maintenance

Preventive Maintenance Overview

Overview of routine maintenance tasks for the Vapro osmometer, including cleaning and reinstalling the TC head.

Removing the TC Head

Step-by-step instructions for safely removing the TC head from the Vapro osmometer for cleaning or service.

Cleaning the TC Head

Detailed procedure for cleaning the TC head, including materials needed and step-by-step instructions.

Reinstalling the TC Head

Instructions for correctly reinstalling the TC head and its connector after cleaning or maintenance.

Equilibration After Cleaning

Explains the thermal equilibrium process after cleaning and reinstalling the TC head.

Severe or Stubborn Contamination

Methods for handling and removing severe or stubborn contamination from the Vapro osmometer's TC head.

Solving Problems

Troubleshooting Guide

Describes problems encountered with the Vapro osmometer and provides suggested solutions via a flowchart.

Common TC Head Problems

Identifies common problems related to the TC head, such as contamination, deformation, or breakage.

External Factors Affecting Precision

Discusses external factors like calibration standards, sampling errors, and micropipettor use that can affect measurement precision.

Deformed or Broken Thermocouple

Details how to identify and potentially restore a deformed thermocouple or the need for replacement.

Instrument Specifications

Accessories, Supplies, and Replacement Parts

Theory of Operation

Equilibration and Zero Set

Explains the first program step of the Vapro osmometer: equilibration and zero set.

Cooling Process

Details the second program step of the Vapro osmometer: cooling via the Peltier Effect.

Dew Point Convergence

Explains the third program step of the Vapro osmometer: dew point convergence.

End of Sequence and Readout

Describes the final program step of the Vapro osmometer, including sequence completion and final readings.

Special Application Notes

Clinical and General Research Applications

Discusses the Vapro osmometer's advantages in clinical and general research applications, including small sample requirements.

Procedure for Very Small Samples

Provides instructions for preparing and measuring samples using very low volumes (under 4 µL).

Measuring Large Samples

Guidance on measuring large samples, considering sample material properties and using alternative sample holders.

Sampling Viscous and Nonhomogeneous Specimens

Techniques for sampling viscous or nonhomogeneous specimens, including methods for ensuring proper sample disc saturation.

Osmometry with Multi-Solvent Solutions

Discusses challenges and considerations when performing osmometry on solutions containing multiple solvents.

Osmolality Standards

Serial Data Output

Setup Menu

WESCOR Vapro 5520 Specifications

General IconGeneral
Measurement PrincipleVapor pressure osmometry
Sample Volume10 µL
Measurement Range0 to 4000 mOsm/kg H₂O
Resolution1 mOsm/kg H₂O
Sample CapacitySingle sample
DisplayLCD
ConnectivityRS-232
Power Requirements100-240 VAC, 50/60 Hz
Accuracy±2 mOsm/kg H2O
Operating Temperature15-30°C (59-86°F)