IOP 011B.9
Effective Date: 1/09/2017
Page 2 of 4
PROCEDURES:
I. Meter preparation
A. At initial set-up six AA-size batteries are installed.
1. Use a screwdriver or coin to remove the thumbscrew on the bottom of the
meter.
2. Open the battery-chamber cover.
3. Install the batteries according to the label on the battery-chamber sleeves.
4. Press and release the ON/OFF button on the front of the instrument. The LCD
will come on; if not, consult the troubleshooting guide.
5. This procedure is also used when LO BAT appears on the LCD.
B. Remove the probe from the calibration chamber and add 6-8 drops of distilled water into
the sponge on the bottom of the chamber. Turn the instrument over to allow excess
water to drain.
C. Prepare the probe: this procedure is used at initial set-up and when membrane
replacement is necessary.
1. Unscrew the probe sensor guard.
2. Remove the O-ring and old membrane (if present).
3. Rinse the sensor tip and KCl reservoir with distilled water.
4. Prepare the electrolyte according to the directions on the KCl solution bottle.
5. Install the membrane (YSI Model 5775 standard membrane; 1 mil
recommended).
a. Secure a membrane between your thumb and the probe body. Add
electrolyte to the probe until a large meniscus completely covers the
gold cathode. Do not touch the center of the membrane.
b. With the thumb and forefinger of your other hand, grasp the free end of
the membrane and with a continuous motion, stretch the membrane up,
over, and down the other side of the sensor. Stretching forms the
membrane to the contour of the sensor tip.
c. Secure the end of the membrane under your forefinger while continuing
to hold the probe.
d. Roll the O-ring over the end of the probe, while being careful not to
touch the membrane surface. Leave no wrinkles in the membrane or
trapped air bubbles under the membrane. Minor wrinkles may be
removed by lightly tugging on the edges of the membrane beyond the
O-ring.
e. Trim excess membrane from the probe with scissors or a sharp
knife. Check that the stainless steel temperature sensor is not covered
by excess membrane.
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