10x Genomics Chromium X Series User Manual

10xgenomics.com
User Guide | CG000396 | Rev G
Chromium X Series (X/iX)
with Readiness Test
For use with:
Chromium X & Accessory Kit, 12-Month Warranty, PN-1000331
Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327
Chromium X & Accessory Kit, 24-Month Warranty, PN-1000332
Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327
Chromium iX & Accessory Kit, 12-Month Warranty, PN-1000328
Chromium iX Accessory Kit, PN-1000323
Chromium iX & Accessory Kit, 24-Month Warranty, PN-1000329
Chromium iX Accessory Kit, PN-1000323
Chromium iX/X Refurbished, PN-1000326R

10x Genomics Chromium X Series Specifications

General

Sample Types	Single cells, nuclei
Data Output	Single-cell gene expression
Compatibility	Compatible with Illumina sequencing platforms
Supported Assays	Single Cell Gene Expression, Single Cell ATAC
Chip Compatibility	Chromium X Chip
Automation	Semi-automated
Software	Cell Ranger, Loupe Browser
Operating Humidity	Standard laboratory conditions
Samples per run	Up to 8 samples

Summary

Notices

Legal Notices

Legal disclaimers and terms of use for the document and products.

Software Update Warranties

Details on instrument and software update warranties and conditions.

Support Contact Information

Contact details for technical support and general inquiries.

Document Revision Summary

General Changes Overview

Summary of broad changes introduced in the current revision.

Specific Changes Detailed

Listing of specific modifications and additions made in this revision.

Introduction to Chromium X Series

Product Identification Features

Describes the identification labels and markings on the instrument.

Product Specifications Summary

Lists the technical specifications and operating parameters of the instrument.

Safety and Compliance

Safety Precautions and Symbols

Outlines essential safety warnings, symbols, and handling precautions.

Regulatory Compliance Standards

Details the certifications and standards the instrument complies with.

System Components Overview

Instrument Component Identification

Illustrates and labels the main external components of the instrument.

Accessory Kit Contents

Lists the items included in various accessory and upgrade kits.

System Operation Guide

Installation and Setup Procedures

Guides through the initial installation, bench space, and power setup.

First-Time Instrument Use

Covers initial power-on, network setup, and registration.

Readiness Test Execution

Details the procedure for performing the initial system readiness test.

Performing a Sample Run

Explains how to load samples and start a main operation run.

Tips and Best Practices

Next GEM Chip Handling

Provides guidance on handling the Chromium Next GEM Chip correctly.

Chip Placement on Tray

Explains how to correctly place the chip and holder onto the instrument tray.

10x Genomics Cloud Usage

Describes the benefits and process of registering instruments to the cloud.

Instrument Menu Options

Menu Overview and Run Logs

Navigating menus, accessing run logs, and exporting data.

Connectivity and Diagnostics

Configuring network settings and enabling diagnostic features.

System Settings and Security

Managing date/time, network security, and system information.

Firmware Update Process

Step-by-step guide for updating instrument firmware.

Instrument Maintenance

System Cleaning and Servicing

Instructions for cleaning the instrument's interior and exterior.

Shipping and Transportation Guidance

Procedures for preparing the instrument for shipping or moving.

Troubleshooting Guide

Error Screen Identification and Handling

Explains how to identify and respond to instrument error messages.