Acea NovoCyte Quick Start Guides

NovoCyte
®
and NovoCyte
®
Quanteon Flow Cytometers
QUICK START & SAMPLE ACQUISITION GUIDE
STARTUP
STARTUP
RUN
SAMPLES
QC TEST
1. Check for adequate levels of the NovoFlow
®
, NovoRinse
®
, and
NovoClean™ in the fluidic containers. Ensure that each is at
least 1/2 full, and preferably empty waste container.
2. Press the power button on the front panel of the flow
cytometer to turn on the instrument. Launch the
NovoExpress
®
software on the computer. Startup is
automated and takes approximately 6 minutes. Check the
instrument status as indicated by the LED indicator on the
front panel:
Green = Normal.
The instrument is ready to use.
Orange = Warning.
Click on the status bar in the NovoExpress software to review
the warning message.
Red = Error.
Click on the status bar to review the error message.
Refer to Section 5: Troubleshooting in the Flow Cytometer
Operator’s Guide.
QC TEST
1. Add 1 drop of the NovoCyte QC particles to 0.5 mL of dilution
buffer (0.4 mL PBS and 0.1 mL NovoRinse solution).
2. On the main menu, click on Instrument tab → Under Operation
Section → Click QC Test and follow the software prompts to
complete the test.
3. If the QC Test results display Pass or Acceptable, the instru-
ment may be used to acquire data. If any QC Test results
display “Fail”, refer to Section 5: Troubleshooting in the Flow
Cytometer Operator’s Guide.
SHUTDOWN
1. Press the power button on the front panel of the flow
cytometer. The instrument will automatically perform the
shutdown cleaning process and then power off.
2. When completed the LED indicator will turn off.
FLUIDICS MANAGEMENT
1. The soware will display a warning message instrucng the
user to add more reagents when the volumes of NovoFlow,
NovoClean, and NovoRinse are less than the set thresholds of
0.9 L, 150 mL, and 150 mL, respecvely.
2. To add reagents:
1) Make sure the instrument is Off or in the Ready status.
2) Disconnect the tubing from the container.
3) Bring the container to the sink. Unscrew the cap and add
more reagent to the container.
4) Screw the cap back on, reconnect the quick coupler, and
return the container back to the fluidics staon. Ensure
that the tubing is not twisted or kinked.
5) If the instrument was in the Ready status, click Instrument
tab → Under Fluidics Maintenance → Click Priming in the
soware to prime the system before running samples.
3. Similarly, the soware will warn the user to empty the waste
container when the waste volume exceeds 2.8 L.
1) Follow the steps above to disconnect the waste container
from the instrument. Dispose of the contents according to
local safety guidelines.
2) Add 300 mL of bleach to the waste container and
reconnect the container to the instrument.
SETUP
SAMPLES AND PLOTS
VERIFY
CORRECT PARAMETERS AND
ACQUISITION SETTINGS
Events/Volume/Flow Rate/Threshold
RUN
COMPENSATION
CONTROLS
(if applicable)
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Acea NovoCyte Specifications

General

Type	Flow Cytometer
Number of Lasers	Up to 3
Fluorescence Channels	Up to 13
Data Acquisition Rate	Up to 35, 000 events/second
Software	NovoExpress
Power Requirements	100-240 VAC, 50/60 Hz
Detection Technology	Fluorescence detection
Lasers	Blue (488 nm), Violet (405 nm)
Sample Input	96-well plate, tubes
Throughput	Up to 35, 000 events per second
Sample Throughput	Up to 96 samples in microplates
Detection	Fluorescence
Cell Size Range	0.5 μm to 50 μm