AmScope ME520 Series - Operation; Setting up; Focusing

8
ME520
Series
Operation
Setting Up
1. Loosen the head lock screw and adjust the microscope head so that it is in a comfortable position for observa-
tion. Lock the head-lock screw.
2. With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyeubes and ro-
tating the eyepiece tubes either towards or away from each other until only one circle of light is seen by both eyes.
3. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly
in place with the metal slide holders (clips) of the mechanical stage.
4. Using the slide controls, center the specimen over the stage opening, lining it up with the light and the objective
lens.
5. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired intensity of
light is achieved.
Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to lo-
cate and focus on the specimen. As you move up in magnification you may need to refocus the image a little each
time.
2. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your other eye. Use
the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.
Note: You may loosen the limit-stop knob (located on the inside of the coarse focusing knob on the left side of the
microscope) in order to give yourself the full range of motion for fine tuning the focus.
4. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for best results.
Note: Please be careful when moving the mechanical stage if you need to re-center the sample, or if moving the
stage very close to the objectives. The limit stop is designed to prevent impact between objective and slide, so when
it is off you will be able to damage the microscope. For safety, when using the 40x objective, engage the limit stop
once you have it close or in contact with the objective.