Bio-Optica WB1770 - User Manual

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Overview

Important Safety Precautions
Graphic Symbols on the Label
Technical Specifications
Installation and Usage
Maintenance
Troubleshooting

The Bio-Optica Water Bath WB1770 (Code: 40-300-000) is a thermostated bath designed for laboratory use, specifically for collecting and drying histological sample sections. It is intended to be used in conjunction with a laboratory microtome, maintaining the water temperature at a desired level to facilitate optimal floating and spreading of sections. The device is marked with CE and IVD symbols, indicating compliance with European safety and in-vitro diagnostic medical device regulations.

Important Safety Precautions

Before using the instrument, it is crucial to read and understand all instructions and warnings in the manual. These provide important information regarding installation, use, and maintenance. Bio-Optica Milano S.p.A. is not responsible for damages resulting from improper or incorrect use, or non-compliance with the manual's prescriptions and safety regulations.

Upon unpacking, ensure the instrument is intact and free from transport damage. Before connecting to the power supply, verify that the instrument's rating matches the electrical distribution network. The WB1770 is designed solely as a water bath for laboratory applications; any other use is considered improper and potentially dangerous. Only authorized and professionally qualified personnel should operate and maintain the instrument. Annual periodic maintenance must be performed by Bio-Optica authorized personnel.

Electrical safety is ensured only when the instrument is correctly connected to an effective earth circuit, as per electrical safety regulations. The instrument is supplied with a 2-wire + earth power cable. Do not remove the chassis or any parts during operation. Always switch off and disconnect the power cable before opening the instrument, an operation reserved for authorized and qualified personnel. To prevent malfunctions, avoid operating the instrument near strong electromagnetic fields or using transmitters like cellular phones nearby. In case of serious malfunction, switch off the instrument and contact Technical Assistance. All waste, including infectious and radioactive materials, must be disposed of according to current regulations. The instrument is marked with the WEEE symbol (2002/96/CE), meaning it should not be disposed of as normal waste but taken to authorized collection centers. The manual's content may change without prior notice, and a declaration of conformity is included.

Graphic Symbols on the Label

The instrument's label, located near the power supply socket, includes several graphic symbols:

REF: Catalogue Number
SN: Serial Number
~: Alternating Current
Fuse symbol: Indicates the fuse
i: Consult the instructions for use
CE: CE Marking
IVD: In Vitro Diagnostic Medical Device
WEEE symbol: Disposal of electric and electronic equipment
Manufacturing date symbol: Date of manufacture
Manufacturer symbol: Manufacturer

Technical Specifications

Dimensional Features:

Dimensions (WxDxH): 350x365x155 mm
Slide-dryer plate dimensions (WxD): 350x100 mm
Weight: 8 Kg

Electrical Connections:

Power supply: 230V~ 50/60Hz
Power: 350 Watt
Fuses: 2 delayed fuses, 3.15 Ampere (T3.15AH250V), 5x20 mm

Structural Features:

Chassis: 15/10 thickness painted sheet steel.
Probe: NTC10K probe with articulated arm and safety device that cuts off power and basin heating when the basin is removed.
Basin: Removable Pyrex basin (approx. 1.2 liters capacity) for easy filling and emptying.
Illumination: A 6 Watt neon lamp illuminates the basin to facilitate section collection.
Slide-dryer plate: Located at the back, capable of holding up to 24 slides.

Technical Features:

Water Bath Temperature Regulation: From +20°C to +70°C via electronic microprocessor thermostat. Reading precision ±1°C.
Temperature Control: Proportional Integral Derivative (PID) control.
Temperature Sensing: NTC10K probe directly immersed in water.
Parameter Modification: Through control panel with decimal digital display.
Slide-Dryer Plate Temperature Regulation: From +20°C to +65°C via analog manual thermostat. The full scale up to 90°C is not usable.

Installation and Usage

Installation: Place the instrument on a flat, stable workbench and connect it to a 230V~ 50/60Hz electrical outlet using the provided cable. Do not use extension cords or adapters, and do not modify the supplied cable.

Filling the Basin:

Pull the probe and rotate it clockwise.
Fill the basin with distilled water up to half its level.
Immerse the probe in the water.
Switch on the instrument by pressing the green main switch. The water temperature will be displayed.

Setting Water Bath Temperature:

Press BUTTON 1 for about one second to view the set temperature.
While holding BUTTON 1, use BUTTONS 2 or 3 to adjust the desired value. A temperature of approximately 40°C is recommended.
Release BUTTON 1 to save the new value.
BUTTON 4 is for technical assistance use only to set parameters. A red LED indicates when the heating resistance is active or inactive.

Changing LAE Thermostat Parameters (Advanced Settings): Operating parameters are preset but can be modified.

To access the setup menu, press BUTTONS 1 and 4 simultaneously for 5 seconds.
Use BUTTONS 2 and 3 to select the parameter to modify.
Press BUTTON 1 to view the parameter's value.
While holding BUTTON 1, use BUTTONS 2 and 3 to set the desired value.
Release BUTTON 1 to save the new value and display the next parameter.
To exit setup, press BUTTON 4 or wait 30 seconds.

Adjustable Parameters:

SPL (Minimum settable temperature): 20°C
SPH (Maximum settable temperature): 70°C
OS1 (Temperature probe calibration): Between +01 and -02

Note on OS1: The average deviation between the probe's reading and the actual temperature is minimal (±1.5°C). To verify, bring the water to 40°C and measure with a digital thermometer. If the display shows 40°C and the thermometer reads 38°C, subtract 2 from OS1 to zero the deviation. Do not modify Pid, 1IT, 1DT, 1AR, 1CT values to ensure optimal PID control.

Setting Slide-Dryer Plate Temperature: The plate heating starts when the main green switch is pressed. To turn it off, rotate the thermostat knob counter-clockwise to its stop. The instrument is factory-tested and preset to approximately 40°C. To adjust:

Switch on the instrument and wait about 15 minutes.
Measure the plate temperature with a thermometer or by drying some slides.
Adjust the temperature by rotating the black knob in small increments, pausing for about 10 minutes between adjustments.

Maintenance

Cleaning: Always switch off the instrument and unplug the power cable before cleaning.

Pyrex basin: Use decalcifying products or acids for limescale, and solvents for paraffin residues.
Chassis: Use only alcohol or non-aggressive detergents. Avoid abrasive products or acids to prevent damage to painted or plastic parts. The instrument does not require sterilization as it is intended for fixed and paraffin-embedded histological samples, not fresh samples.

Periodic Maintenance: Annual periodic maintenance must be performed by Bio-Optica qualified and authorized personnel. Contact Technical Assistance at 02-21271310 for information.

Opening the Instrument: Switch off the instrument and unplug the power cable. Remove the screws from the lower panel (newer models use TORX screws with a central hole). Place the instrument back on its feet and rotate the upper part to the left.

Replacing Fuses: If the instrument does not turn on, check the power cable connection, power supply, and ensure the two fuses under the power socket are not blown. Replace with fuses of the same value if necessary.

Procedure: Switch off and unplug the instrument. Gently press the small plastic cover over the fuses (use a small screwdriver if needed), replace the fuses, close the cover, and check if the instrument turns on.

Replacing the Lamp: Switch off and unplug the instrument. Remove the bottom panel by unscrewing the 8 fixing screws. Lift the chassis by rotating it to the left. Rotate the lamp 90° and lift it out. Replace it with an identical model and rotate it 90° back into place. Close the instrument.

Replacing LAE Thermostat: Switch off and unplug the power cable, then open the instrument. The thermostat is secured by clips or locking brackets. The wiring connections are indicated on the label on the upper side of the thermostat.

Troubleshooting

Instrument doesn't switch on:

Check power cable connection, power supply, and fuse functionality. Replace fuses if necessary.

Instrument lights up but keys don't respond:

The membrane covering the keys might warp and block them over time. Replace the LAE thermoregulator.

Instrument lights up but thermostat doesn't light up and resistance doesn't heat:

The "L"-shaped stainless steel arm holding the temperature probe must close a microswitch to power the thermostat. Verify microswitch function, positioning, and internal disk fixing.

Error signal (e.g., DL1) appears on display:

Verify correct connection of the temperature probe.

Basin doesn't heat:

The thermostat indicates circuit closure with a red dot, powering the resistance. If the red LED doesn't light up, check thermostat parameters. If it lights up, check power supply and heating resistance function.

Slide-dryer upper plate doesn't heat: