BIO RAD CFX96 Touch User Manual

Stand-Alone Operation
The CFX96 Touch system can also be operated as a stand-alone instrument that can
run a real-time PCR protocol without a computer attached. However, data analysis
requires the use of CFX Manager software installed on a PC.
Creating a Protocol
n
To create a new run, touch New Protocol in the home screen to open a
New Protocol template (Figure 10); touch the Name field, then enter a protocol
name to save
n
To edit an existing protocol, touch Saved Files to open the file library
and select a protocol to edit
n
For more information about creating, editing, and running protocols, refer to the
CFX96 Touch and CFX384 Touch systems instruction manual
Exporting Data
When a run is finished, the fluorescence data need to be transferred to a computer
running CFX Manager software for analysis. At the end of a run, the data file is
automatically saved to the Real-Time Data folder located in the Real-Time Data
directory (Figure 11). Up to 100 real-time PCR runs are stored in this folder.
Exporting Data Using a USB Flash Drive
n
If a USB flash drive is present in a USB port on the C1000 Touch chassis at the end
of a run, the data file (.zpcr) will automatically be saved to the Real-Time Data folder,
which will be created on the USB flash drive
n
If a USB flash drive has not been inserted into a USB port, follow these instructions
to transfer a data file to the USB flash drive:
1. Touch Saved Files in the home screen to access the Files folders.
2. In the Locations pane, select Real-Time Data.
3. Select the file to export from the Files pane. Information about the selected file will
be displayed in the Preview pane.
4. To export the file, touch the File Options button.
5. Touch the OK button to save the file to the attached USB flash drive.
Exporting Data Using Email
You can choose to email your data from the C1000 Touch chassis after configuring
your email settings (see the Setting Up Email on the C1000 Touch Chassis section).
To send an email with an attached data file (.zpcr) from the C1000 Touch chassis at
the end of a run, enter an email address in the Additional Settings window prior to the
run start (Figure 12).
Note: When users are logged in, they can specify that a notification and an
attachment be sent to a particular email address at the completion of each run.
Fig. 11. Real-Time Data folder stores
real-time PCR runs.
Fig. 10. Default real-time PCR protocol.
Fig. 12. Selecting email notification.

BIO RAD CFX96 Touch Specifications

General

Instrument Type	Real-Time PCR Detection System
Sample Capacity	96-well plate
Detection Method	Fluorescence
Excitation Source	LED
Detection Channels	5
Temperature Range	4°C to 100°C
Temperature Accuracy	±0.2°C
Temperature Uniformity	±0.4°C
Thermal Uniformity	±0.4°C
Number of Channels	5
Connectivity	USB, Ethernet
Software	CFX Maestro Software
Ramp Rate	5°C/second
Reaction Volume	10–50 µL
Supported Dyes	FAM, Texas Red, Cy5
Optical System	LED
Power Requirements	100–240 V, 50/60 Hz