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General| Type | Real-Time PCR Detection System |
|---|---|
| Detection Channels | 4 |
| Temperature Accuracy | ±0.5°C |
| Format | 96-well |
| Thermal Accuracy | ±0.5°C |
| Sample Capacity | 96-well |
| Data Acquisition | Real-time |
| Thermal Range | 0°C to 100°C |
| Temperature Range | 0°C to 100°C |
Lists the components included in the iQ Optical Module retrofit kit and pre-installed items.
Steps for removing the standard lid and installing the optical lid on the iCycler reaction module.
Instructions for attaching the support bracket to the rear of the iCycler thermal cycler.
Procedure for attaching the Optical Module onto the iCycler's optical lid.
Details on system cabling, including optical, reaction, and iCycler module connectors.
Guidance on installing, removing, and positioning filters and filter blanks in the filter wheels.
Step-by-step instructions for installing the iCycler iQ software from the installation CD-ROM.
Procedure to update the iCycler's firmware for compatibility with the iQ Detector.
Steps to capture an image of a calibration plate using iQconfig for mask alignment.
Procedure for adjusting the DC offset to ensure correct pixel intensity and image quality.
Guide to aligning mask regions over individual wells using the Mask Editor utility.
Instructions for preparing a calibration plate with appropriate fluorescein concentrations.
Steps for running the prepared calibration plate through a temperature cycling protocol.
Guidance on optimizing image capture settings for collecting well factors.
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