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BIO RAD CFX96 - User Manual

BIO RAD CFX96
158 pages
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CFX96
and CFX384
Real-Time
PCR Detection Systems
Instruction Manual
Catalog # 184-5384
# 185-5384
# 184-5096
# 185-5096

Table of Contents

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Summary

Safety and Regulatory Compliance

Safety Warning Labels

Labels warning about injury or harm from instrument use.

Instrument Safety Warnings

Warnings about instrument use and potential hazards.

Safe Use Specifications and Compliance

REGULATORY COMPLIANCE

Details compliance with safety and electromagnetic standards.

Chapter 1. System Installation

Unpacking the Optical Reaction Module

Instructions for unpacking the optical reaction module.

System Requirements

Specifies power sources and environmental conditions for system operation.

Setting up the system

Provides steps for physically installing and setting up the instrument.

Installing CFX Manager Software

Guides on installing the necessary software for instrument control and analysis.

Installing the Drivers

Steps to install necessary system drivers for software-controlled operation.

Chapter 2. Introduction to CFX Manager Software

Detected Instruments Pane

Instrument Properties Window

Chapter 3. Running Experiments

Experiment Setup Window

Interface for setting up and configuring experiments.

Protocol Tab

Section for selecting, creating, or editing experimental protocols.

Plate Tab

Section for selecting, creating, or editing experimental plate layouts.

Start Run Tab

Final configuration before starting an experiment, including instrument selection.

Run Details Window

Window to monitor the progress and status of an experiment run.

Chapter 4. Protocols

Protocol Editor window

Interface for creating and editing PCR protocols.

Temperature Control Mode

Explains the two temperature control modes used by the instrument.

Protocol AutoWriter

Tool for automatically generating PCR protocols.

Chapter 5. Plates

Plate Size and Type

How to select appropriate plate size and type for experiments.

Scan Mode

How to select scan modes for collecting fluorescence data.

Select Fluorophores Window

Window to select fluorophores for analysis.

Well Loading Controls

Controls for specifying well contents and sample information.

Chapter 6. Stand-Alone Operation

Control Panel

Describes the instrument's control panel interface and keys.

Experiment Setup

How to set up and run experiments directly on the instrument.

Chapter 7. Data Analysis Overview

Chapter 8. Data Analysis Windows

Quantitation Tab

Tab for setting analysis conditions and viewing amplification data.

Chapter 9. Gene Expression Analysis

Gene Expression

How to normalize relative differences in target concentration between samples.

Plate Setup for Gene Expression Analysis

Requirements for setting up wells for gene expression analysis.

Gene Expression Tab

Displays relative expression of targets in charts and spreadsheets.

Normalized Gene Expression

How to normalize data using reference genes for relative expression analysis.

Relative Quantity

Method to quantitate samples without reference genes.

Gene Expression Calculations

Explains the formulas used for gene expression analysis calculations.

Chapter 10. Users and Preferences

Chapter 11. Resources

Instrument Maintenance

Guidelines for maintaining the instrument's performance and longevity.

Cleaning the Optical Reaction Module

Procedures for cleaning the optical reaction module and its components.

Troubleshooting

Solutions for common software and instrument communication problems.

Installing the Software Manually

Steps to manually install the CFX Manager software.

Installing the Drivers Manually

Steps to manually install system drivers for the instrument.

Summary

Safety and Regulatory Compliance

Safety Warning Labels

Labels warning about injury or harm from instrument use.

Instrument Safety Warnings

Warnings about instrument use and potential hazards.

Safe Use Specifications and Compliance

REGULATORY COMPLIANCE

Details compliance with safety and electromagnetic standards.

Chapter 1. System Installation

Unpacking the Optical Reaction Module

Instructions for unpacking the optical reaction module.

System Requirements

Specifies power sources and environmental conditions for system operation.

Setting up the system

Provides steps for physically installing and setting up the instrument.

Installing CFX Manager Software

Guides on installing the necessary software for instrument control and analysis.

Installing the Drivers

Steps to install necessary system drivers for software-controlled operation.

Chapter 2. Introduction to CFX Manager Software

Detected Instruments Pane

Instrument Properties Window

Chapter 3. Running Experiments

Experiment Setup Window

Interface for setting up and configuring experiments.

Protocol Tab

Section for selecting, creating, or editing experimental protocols.

Plate Tab

Section for selecting, creating, or editing experimental plate layouts.

Start Run Tab

Final configuration before starting an experiment, including instrument selection.

Run Details Window

Window to monitor the progress and status of an experiment run.

Chapter 4. Protocols

Protocol Editor window

Interface for creating and editing PCR protocols.

Temperature Control Mode

Explains the two temperature control modes used by the instrument.

Protocol AutoWriter

Tool for automatically generating PCR protocols.

Chapter 5. Plates

Plate Size and Type

How to select appropriate plate size and type for experiments.

Scan Mode

How to select scan modes for collecting fluorescence data.

Select Fluorophores Window

Window to select fluorophores for analysis.

Well Loading Controls

Controls for specifying well contents and sample information.

Chapter 6. Stand-Alone Operation

Control Panel

Describes the instrument's control panel interface and keys.

Experiment Setup

How to set up and run experiments directly on the instrument.

Chapter 7. Data Analysis Overview

Chapter 8. Data Analysis Windows

Quantitation Tab

Tab for setting analysis conditions and viewing amplification data.

Chapter 9. Gene Expression Analysis

Gene Expression

How to normalize relative differences in target concentration between samples.

Plate Setup for Gene Expression Analysis

Requirements for setting up wells for gene expression analysis.

Gene Expression Tab

Displays relative expression of targets in charts and spreadsheets.

Normalized Gene Expression

How to normalize data using reference genes for relative expression analysis.

Relative Quantity

Method to quantitate samples without reference genes.

Gene Expression Calculations

Explains the formulas used for gene expression analysis calculations.

Chapter 10. Users and Preferences

Chapter 11. Resources

Instrument Maintenance

Guidelines for maintaining the instrument's performance and longevity.

Cleaning the Optical Reaction Module

Procedures for cleaning the optical reaction module and its components.

Troubleshooting

Solutions for common software and instrument communication problems.

Installing the Software Manually

Steps to manually install the CFX Manager software.

Installing the Drivers Manually

Steps to manually install system drivers for the instrument.

BIO RAD CFX96 Specifications

General IconGeneral
Temperature Uniformity±0.4 °C
SoftwareCFX Maestro Software
Detection MethodReal-time PCR (qPCR)
Sample Capacity96-well plate
Excitation SourceLED
Ramp RateUp to 5°C/sec
Heating Rate5°C/sec
SensitivitySingle copy detection
Power Requirements100-240 V, 50/60 Hz
Temperature Range0–100 °C

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