Gene Expression Analysis
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4. Fill in the text for the report by entering text and images in option panes (Figure 107).
Figure 107. Example of Header and Logo options in a Gene Study report.
5. Click the Update Report button to update the report preview pane.The report preview
pane shows a view of the Report.
6. Print or save the report. Click the Print button in the toolbar to print the current report.
Select File > Save to save the report as a PDF (Adobe Acrobat Reader file), MHT
(Microsoft document), or MHTML (Microsoft document) formatted file and select a
location to store the file. Select File > Save As to save the report with a new name or in a
new location.
7. Create a report template once you create a report with the content you want to include in
all reports. To create a template, select Te mp la t e > S av e or Save As and save the
current report as a template.
Frequently Asked Questions
The following list is a series of questions (Q) and answers (A) about gene expression analysis in
CFX Manager software.
Q: Why should I normalize my data?
A: Relative quantity data that is not normalized by some means is difficult to interpret. Imagine
the case where you load 1 μg of RNA in one well and 10 ng in the other well. If you perform a
relative quantity analysis on the results from such an assay, then the fact that the 10 ng sample
has a smaller relative quantity value is irrelevant. It is likely the result of using less RNA and not
the result of some biological response.
Q: Why does the formula for relative quantity when a control is selected vary from that outlined
in the geNorm web site?
Inter-Run Calibration Inter-run calibration data
Table 43. Categories for a Gene Study report (continued)
Category Option Description
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