CFX96 and CFX384 Systems Manual
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Figure 24 shows the default end point protocol.
Figure 24. End Point Only protocol.
To change the step temperature or sample volume for the end point only run, click the Start
Run tab and edit the Step Temperature or Sample Volume. Figure 25 shows the step
temperature is changed to 55.0oC.
Figure 25. Change the sample volume on the Start Run tab.
Plate Tab
The Plate tab shows a preview of the selected plate file loaded in the Experiment Setup
(Figure 26). In a real-time PCR experiment, the plate file contains a description of the contents
of each well, the scan mode, and the plate type. CFX Manager software uses these
descriptions for data collection and analysis.
Select one of the following options to select an existing plate, create a new plate, or edit the
currently selected plate:
• Create New button. Open the Plate Editor to create a new plate
• Select Existing button. Open a browser window to select and load an existing plate file
(.pltd extension) into the Plate tab
• Express Load pull-down menu. Quickly select a plate to load it into the Plate tab
TIP: To add or delete plates in the Express Load menu, add or delete files (.pltd
extension) in the ExpressLoad folder. To locate this folder, select Too ls > Us er
Data Folder in the menu bar of the main software window.
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