BIO RAD CFX96 User Manual

CFX96 and CFX384 Systems Manual
1
1 System Installation
Read this chapter for information about setting up the CFX96 or CFX384 system:
• Unpacking the optical reaction module (below)
• System requirements (page 1)
• System overview (page 2)
• Setting up the system (page 3)
• Installing CFX Manager software (page 6)
• Running experiments (page 9)
Unpacking the Optical Reaction Module
Your CFX96 or CFX384 optical reaction module shipment includes these components:
• Optical reaction module
•USB cable
• CFX Manager
Tm
software installation CD
• Instruction manual
• CFX Manager software uick guides for system installation, protocol, plate, data
analysis, and gene expression analysis
Remove all packing materials and store them for future use. If any items are missing or
damaged, contact your local Bio-Rad office.
System Requirements
To operate the CFX96 or CFX384 system, use the following power sources and cables:
• Input power. 100—240 VAC, 50—60 Hz
• Indoor use. Ambient temperature of 15—31
o
C. Relative humidity maximum of 80%
(non-condensing)
• USB cable. If the CFX96 system is going to be controlled by a computer via a USB
cable, the provided cable from Bio-Rad is sufficiently shielded for use.
NOTE: For a full list of the safety and compliance requirements for this instrument,
see “Safety and Regulatory Compliance” on page iii.

BIO RAD CFX96 Specifications

General

Temperature Uniformity	±0.4 °C
Software	CFX Maestro Software
Detection Method	Real-time PCR (qPCR)
Sample Capacity	96-well plate
Excitation Source	LED
Ramp Rate	Up to 5°C/sec
Heating Rate	5°C/sec
Sensitivity	Single copy detection
Power Requirements	100-240 V, 50/60 Hz
Temperature Range	0–100 °C