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Introduction to CFX Manager Software

• Create a new Experiment (page 21). Set up the protocol and plate to begin a new

experiment.

NOTE: Select the appropriate instrument in the pull down list to make sure the

default plate settings match the instrument to be used for the experiment.

• Repeat an Experiment. Set up an experiment with the protocol and plate from a

completed run. If needed, you can edit the experiment before the run

• Open a Data File (page 69). Open a data file to analyze results

• Open a Gene Study (page 101). Open a multi-file gene expression study to analyze

results from multiple gene expression experiments

• Open User Preferences (page 121). Open the User Preferences window to customize

software settings

Detected Instruments Pane

CFX Manager software runs these 1000-series instruments:

• CFX96 and CFX384 real-time PCR systems. Systems include a C1000 thermal cycler

and an optical reaction module

• C1000 thermal cycler. The most versatile of the instruments run by CFX Manager

software. Add and remove these PCR reaction modules to the C1000 chassis in

seconds:

• 96-well fast reaction module, gradient enabled

• 384-well reaction module

• Dual 48/48 fast reaction module, gradient enabled

• S1000 thermal cycler connected to a C1000 thermal cycler. For more information,

see S1000 and C1000 thermal cycler instruction manuals

TIP: Locate PDF copies of the instruction manuals by opening the Documents

folder on the CFX Manager software installation CD.

You can simultaneously run up to four instruments with CFX Manager software, including a

combination of CFX96 system and CFX384 systems, or a mix of real-time PCR systems and

thermal cyclers.

Viewing Detected Instruments

Connected instruments appear in the Detected Instruments pane (page 17). This list shows

each instrument as an icon named with the serial number (default). The list of instruments also

shows individual blocks (Block A and Block B) for each dual block reaction module.

TIP: Right-click any instrument in the Detected Instruments pane to open the

Instrument Properties window and rename the instrument.

Figure 18 shows four detected instruments:

• One C1000 thermal cycler (C48FSIM00) with a dual 48/48 reaction module

• One S1000 thermal cycler (S96FSIM01) with a 96-well block, which is connected to

the C1000 thermal cycler called C48FSIM00

• One CFX384 system (CFX384SIM03)

BIO RAD CFX96 User Manual

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