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BIO RAD CFX96 User Manual

BIO RAD CFX96
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CFX96 and CFX384 Systems Manual
3
Inner lid with heater plate. The heater lid maintains temperature on the top of the
consumable to prevent sample evaporation. Avoid touching or otherwise contaminating
the heater plate. Never poke anything through the holes; the optics shuttle system could
be damaged
•Block. Load samples in this block before the run
Close button. Press this button on the inside of the lid to close the motorized lid
WARNING! Prevent contamination of the instrument by spills, and never run a
reaction with an open or leaking sample lid. For information about general cleaning
and maintenance of the instrument, see “Instrument Maintenance” (page 133).
The back panel of the C1000 chassis includes these features (Figure 3):
Power switch. Press the power switch to turn on the power to the system
Power input. Plug in the power cord here
Ethernet port. Connect an ethernet cable to email run logs and stand-alone data files
USB connections. Use these ports to connect the CFX96 system or CFX384 system to
a computer or to connect an S1000
Tm
thermal cycler
Figure 3. Back panel of C1000 thermal cycler.
WARNING! Avoid contact with the back panel of the C1000 during operation.
Setting up the system
The CFX96 or CFX384 real-time PCR detection system should be installed on a clean, dry,
level surface with sufficient cool airflow to run properly. The CFX96 system or CFX384 system
can be run in two modes: stand-alone or software-controlled. If you are running the system
under software-controlled mode, make sure there is sufficient space for a computer during
setup.
To insert the CFX96 or CFX384 optical reaction module into the reaction module bay of
the C1000 chassis, follow these instructions:
1. Place the C1000 chassis in a suitable location with the locking bar down. Remove any
previously installed reaction modules.
Power
Power
input
switch
USB connections
Ethernet
port

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BIO RAD CFX96 Specifications

General IconGeneral
Temperature Uniformity±0.4 °C
SoftwareCFX Maestro Software
Detection MethodReal-time PCR (qPCR)
Sample Capacity96-well plate
Excitation SourceLED
Ramp RateUp to 5°C/sec
Heating Rate5°C/sec
SensitivitySingle copy detection
Power Requirements100-240 V, 50/60 Hz
Temperature Range0–100 °C

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