BIO RAD CFX96 Touch User Manual

AMPLIFICATION
CFX96 Touch
™
Real-Time PCR System
Installation Quick Guide
Unpacking the Instruments
The CFX96
™
optical reaction module and the C1000 Touch
™
thermal cycler chassis are shipped in separate containers.
Unpack each box carefully. The included power cable, USB
cable, and CFX Manager
™
software CD are required for
installation. The CFX96 optical reaction module may also be
placed on a previously purchased C1000 Touch thermal cycler
for a quick upgrade to a real-time PCR detection system.
Important: Carefully read the safety information and
instrument operating specifications provided in the CFX96
Touch
™
and CFX384 Touch
™
Real-Time PCR Detection
Systems Instruction Manual before using the system.
Setting Up the CFX96 Touch Real-Time PCR System
The CFX96 Touch real-time PCR detection system should
be installed on a clean, dry, level surface with sufficient
cool airflow to run properly.
To insert the CFX96 optical reaction module into the
reaction module bay of the C1000 Touch chassis:
1. Place the C1000 Touch chassis in a suitable location
with the locking bar down.
2. Remove the instructions overlay from the C1000 Touch
chassis. Lift the CFX96 module using the handle
indents above the side air vents, as shown in Figure 1.
3. Position the CFX96 module in the reaction module bay
of the C1000 Touch chassis, leaving about 2 cm of
space in the front.
4. Reach around the thermal cycler and pull up the
locking bar (Figure 2) until it is flush with the sides of
the module bay. This action moves the reaction module
forward, locking it into place (Figure 3).
5. Use the supplied power cord to plug the chassis into
an appropriate electrical outlet.
6. Turn on the CFX96 Touch system using the power
switch on the back of the chassis.
Removing the Red Shipping Screw
The CFX96 optical reaction module is shipped with a
red screw in place in the inner lid in the hole corresponding
to well A1 (see Figure 4). Follow the instructions on the
C1000 Touch screen to remove this shipping screw before
beginning operation.
Fig. 3. The CFX96 module positioned on
the C1000 Touch chassis.
Locking bar
Fig. 1. Lifting the CFX96 optical reaction module
onto the C1000 Touch thermal cycler chassis.
Fig. 2. Locking the CFX96 module into place.
C1000 Touch thermal
cycler chassis
CFX96 optical
reaction module
Fig. 4. Red shipping screw included with
the CFX96 optical reaction module.

BIO RAD CFX96 Touch Specifications

General

Instrument Type	Real-Time PCR Detection System
Sample Capacity	96-well plate
Detection Method	Fluorescence
Excitation Source	LED
Detection Channels	5
Temperature Range	4°C to 100°C
Temperature Accuracy	±0.2°C
Temperature Uniformity	±0.4°C
Thermal Uniformity	±0.4°C
Number of Channels	5
Connectivity	USB, Ethernet
Software	CFX Maestro Software
Ramp Rate	5°C/second
Reaction Volume	10–50 µL
Supported Dyes	FAM, Texas Red, Cy5
Optical System	LED
Power Requirements	100–240 V, 50/60 Hz

Summary

Unpacking and Initial Setup

Unpacking the Instruments

Instructions for unpacking the CFX96 optical reaction module and C1000 Touch thermal cycler chassis.

Setting Up the CFX96 Touch System

Steps to install the CFX96 optical reaction module onto the C1000 Touch chassis.

Removing the Red Shipping Screw

Guidance on removing the shipping screw located in the CFX96 optical reaction module.

Software and Driver Installation

Installing CFX Manager Software

Procedure for installing the CFX Manager software on a compatible computer system.

Installing the Instrument Drivers

Steps to install the necessary drivers for the CFX96 Touch system using the Found New Hardware Wizard.

Run Operations and Data Handling

Creating, Starting, and Analyzing Runs

Guide to using the Run Setup window for protocols, plates, and starting real-time PCR runs.

Stand-Alone Operation and Protocol Creation

Operating the system without a computer and creating new PCR protocols.

Exporting Data Options

Methods for exporting real-time PCR data via USB flash drive or email.

Analyzing a Data File

Process for converting and analyzing stand-alone run data files using CFX Manager.

Setting Up Email Notifications

Configuration steps for outgoing email settings on the C1000 Touch chassis.