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BIO RAD CFX96 User Manual

BIO RAD CFX96
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CFX96 and CFX384 Systems Manual
31
4 Protocols
Read the following chapter for information about creating and editing protocol files:
Protocol Editor window (below)
Protocol Editor controls (page 33)
Temperature control mode (page 37)
Protocol AutoWriter (page 37)
Protocol Editor Window
A protocol instructs the instrument to control the temperature steps, lid temperature, and other
instrument options. Open the Protocol Editor window to create a new protocol or to edit the
protocol currently selected in the Protocol tab. Once a Protocol is created or edited in the
Protocol Editor, click OK to load the protocol file into the Experiment Setup window and run it.
Opening the Protocol Editor
To open the Protocol Editor, follow one of these options:
To create a new protocol, select File > New > Protocol or click the Create New
button in the Protocol tab (page 22)
To open an existing protocol, select File > Open > Protocol, or click the Open
Existing button in the Protocol tab (page 22)
To edit the current protocol in the Protocol tab, click the Edit Selected button in the
Protocol tab (page 22)
TIP: To change the default settings in the Protocol Editor window, enter the
changes in the Protocol tab in the user Preferences window (page 124)
Protocol Editor Window
The Protocol Editor window (Figure 32) includes the following features:
•Menu bar. Select settings for the protocol
Toolbar. Select options for editing the protocol
•Protocol. View the selected protocol in a graphic (top) and text (bottom) view. Click the
temperature or dwell time in the graphic or text view of any step to enter a new value

Table of Contents

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BIO RAD CFX96 Specifications

General IconGeneral
Temperature Uniformity±0.4 °C
SoftwareCFX Maestro Software
Detection MethodReal-time PCR (qPCR)
Sample Capacity96-well plate
Excitation SourceLED
Ramp RateUp to 5°C/sec
Heating Rate5°C/sec
SensitivitySingle copy detection
Power Requirements100-240 V, 50/60 Hz
Temperature Range0–100 °C

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