BIO RAD CFX96 User Manual

CFX96 and CFX384 Systems Manual
141
Index
A
Add Repeats, 27
Adding
Beep
, 37
Extend, 37
Increment, 37
Ramp rate, 37
Repeats in run, 27
Adjusting
Allelic Discrimination data
, 94
Data analysis charts, 78
End point data, 93
Gene expression data, 104
Melt Curve Data, 88
Threshold, 74
Trace color, 82
Trace style, 82
All Channels scan mode, 45, 60
Allelic Discrimination, 93
Adjusting data, 94
RFU, 93, 96
Spreadsheet, 95
Amplicon length, 38, 39
Analysis Mode, 75
Annealing temperature
Protocol AutoWriter
, 38, 39
Auto Efficiency, 50, 108
B
Back panel, 3
Balance
Cut microplates
, 10
How to, 10
Tube strips, 10
Baseline
Window
, 74
Baseline Subtracted
Curve Fit mode
, 75
Baseline Subtracted mode, 75
Baseline Threshold window, 74
Beep, 37
Bio-Rad Laboratories
Contact
, ii
Resources, ii
Web site, ii
Block, 3
Mode, 37
Button, 27
Clear Replicate #, 48
Clear Wells, 48
Close lid, 3, 26
Delete step, 37
Experiment Settings, 48
Flash Block Indicator, 27
Open lid, 26
Pause, 27
Protocol AutoWriter, 37
Protocol Editor, 33
Resume, 27
Show Analysis Settings, 50, 107
Skip Step, 27
C
C1000 Control Panel, 53
Cable, catalog number, 138
Calculated mode, 37
Calibration

BIO RAD CFX96 Specifications

General

Temperature Uniformity	±0.4 °C
Software	CFX Maestro Software
Detection Method	Real-time PCR (qPCR)
Sample Capacity	96-well plate
Excitation Source	LED
Ramp Rate	Up to 5°C/sec
Heating Rate	5°C/sec
Sensitivity	Single copy detection
Power Requirements	100-240 V, 50/60 Hz
Temperature Range	0–100 °C