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BIO RAD CFX96 User Manual

BIO RAD CFX96
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Index
142
Inter-run in a Gene Study
, 109
Cancelling a run, 27
Catalog numbers
Instruments
, 138
PCR supermixes, 138
Reaction modules, 138
Reverse transcriptase, 138
Software, 138
USB cable, 138
CFX Manager
Files
, 20
CFX96 system, 2
Overview, 2
Changing
Baseline
, 74
Sample volume, 23, 33
Thresholds, 74
Chart
Data analysis
, 78
Clear Replicate #, 48
Clear Wells button, 48
Clearing wells in Plate Editor, 78
Close button for lid, 3
Close Lid button, 26
Closing the lid, 26
Coefficient of Variation, 105
Collection Name, 48, 107
Color, 45, 50, 108
Components in shipment, 1
Concentration, 46, 47
Contact Bio-Rad Laboratories, ii
Control
Relative quantity with
, 116
Creating
Data report
, 98
Protocol, 39
Well groups, 50
D
-d(RFU)/dT
Spreadsheet
, 91
Data
Files
, 20
Data analysis
About
, 69
Adjusting, 69
Charts, adjusting, 78
Clearing wells, 78
Data analysis, 93
End Point, 91
Excluding wells, 76
Formula, 115
Gene Expression, 101
Melt Curve, 87
Menu bar, 71
Plate content, 69
Toolbar, 70
Well contents, 69
Well groups, 50
Well selector, 75
Data file
Report
, 97
Data report
Creating
, 98
Delete Step button, 37
Deleting a step, 37
Detected Instruments, 12
Dilution Series, 48
E
Email function on the C1000, 62
Email Setup in CFX Manager, 123
Email setup on the C1000, 65
Email Tab, 123
End Point
Adjusting data
, 93
Changing sample volume in protocol, 23
RFU data analysis, 91
Spreadsheet, 93
Tab, 92
End Point Only Run protocol, 22
Enzyme type, 38, 39
Exclude well using right click option, 76
Excluding wells
Plate Editor
, 76
Experiment Settings, 48
Auto Efficiency, 50, 108
Button, 48
Color, 50, 108
Show Analysis Settings button, 50, 107
Show Graph, 50, 108
Targets tab, 49, 107
Export All Data Sheets to Excel, 71
Export Template, 52
Exporting Stand-Alone Data, 61
Express Load
Protocol
, 22, 23
Extend, 37
F
Files
Data
, 20

Table of Contents

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BIO RAD CFX96 Specifications

General IconGeneral
Temperature Uniformity±0.4 °C
SoftwareCFX Maestro Software
Detection MethodReal-time PCR (qPCR)
Sample Capacity96-well plate
Excitation SourceLED
Ramp RateUp to 5°C/sec
Heating Rate5°C/sec
SensitivitySingle copy detection
Power Requirements100-240 V, 50/60 Hz
Temperature Range0–100 °C

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