BIO RAD CFX96 User Manual

CFX96 and CFX384 Systems Manual
105
• Highest expression. Scale the normalized gene expression to the highest for each
target by dividing the expression level of each sample by the highest level of expression
in all the samples. This scaling option uses the scaled to highest formula
• Lowest expression. Recalculate the normalized gene expression for each target by
dividing the expression level of each sample by the lowest level of expression in all the
samples. This scaling options uses the scaled to lowest formula
ERROR TYPE
Select an option for the type of error calculations (error bars) in the Gene Expression graph:
• Standard Error of the Mean (default, SEMs)
• Standard Deviation (Std Devs)
CHART ERROR BAR MULTIPLIER
Select a multiplier for the error bars in the Gene Expression graph. Select one of these
integers: +/- 1 (default), 2, or 3. The type of multiplier changes when you select the Error Type:
• SEMs for Standard Error of the Mean
• Std Devs for Standard Deviations
TARGET STABILITY VALUE
Open this window whenever more than 1 reference gene is used. The software calculates two
quality parameters for the reference genes:
• Coefficient of Variation (CV) of normalized reference gene relative quantities. Lower
CV values denotes higher stability
• M-value. A measure of the reference gene expression stability
Right-Click Menu Options for Gene Expression Graph
Right-click on the Gene Expression graph to select the items shown in Table 37.
Table 37. Right-click menu items
Item Function
Copy Copy the chart to a clipboard
Save as Image Save the graph in the chart view as an image file. The default
image type is PNG. The other selections for image file types
include GIF, JPG, TIF, and BMP
Page Setup... Select a page setup for printing
Print... Print the chart view
Show Point Values Display the relative quantity of each point on the graph when
you place the cursor over that point
Set Scale to Default Set the chart view back to the default settings after magnifying
it
Chart Options... Open the Chart Options window to adjust the graph
Sort Sort the order that samples or targets appear on the chart X-
axis
User Corrected Std Devs Calculate the error bars using the corrected standard deviation
formula
Use Solid Bar Colors Display solid bars in the graph

BIO RAD CFX96 Specifications

General

Temperature Uniformity	±0.4 °C
Software	CFX Maestro Software
Detection Method	Real-time PCR (qPCR)
Sample Capacity	96-well plate
Excitation Source	LED
Ramp Rate	Up to 5°C/sec
Heating Rate	5°C/sec
Sensitivity	Single copy detection
Power Requirements	100-240 V, 50/60 Hz
Temperature Range	0–100 °C