CFX96 and CFX384 Systems Manual
143
Gene Study
, 20
Plate, 20
Protocol, 20
Software, 20
Flash Block Indicator button, 27
Fluorophores, 46
Formula, 116
Data analysis, 115
E (see reaction efficiency), 84
Normalized expression, 117, 119
Normalized expression, scaled, 118
Reaction Efficiency (E), 84
Relative quantity, 116
SD normalized expression, 119
Standard error, 119
Standard error normalized expression, 119
FRET, 45, 60
Front view, 2
G
Gene expression
Adjusting data
, 104
Data analysis, 101
Graph data, 104
Normalizing data, 103
Relative quantity, 104
Right-click for graph, 105
Scaling options, 104
Spreadsheet, 106
Tab, 102
Gene Expression Gateway, ii
Gene Study, 109
Files, 20
Inter-run calibration, 109
Preparing data, 110
Report, 113
Show Details, 113
Study Analysis tab, 109, 111
Study Setup tab, 110
Glossary
Software
, 135
GOI
Normalization factor
, 117
Normalized expression, 117, 118, 119
Relative quantity, 116
SD Normalized expression, 119
GOTO
Adding repeats
, 27
Inserting, 35
Gradient
Inserting
, 34
Step, 34
Gradient Calculator, 32
Guides
Quick Guides
, 11
Software Help, 11
H
Heated plate, 3
Help tools, 135
Software, 135
I
Import Template, 52
Increment, 37
Inner lid, 2
Instruments
Safety
, iii
Warning labels, iii
Inter-run calibration, 109
L
Layout
Quantitation tab
, 72, 82
LED on lid, 2
Library, 46
Lid
Green LED
, 2
Opening, 26
Life Science Research web site, ii
M
Magnification in Plate Editor, 44
Main software window, 11
Melt Curve
Adjusting data
, 88
Data analysis, 87
Inserting, 35
Opening tab, 87
RFU data, 87
Spreadsheet, 88
Melt Curve Data, 90
Amplification Data (RFU), 90
Plate, 90
Tab, 89
Melt Peaks
Spreadsheet
, 89