Gene Expression Analysis
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Figure 104 shows the Gene Study window, including the Study Setup and Study Analysis tabs.
Figure 104. Gene Study window.
Study Setup Tab
Before importing data into a Gene Study, do the following in the Data Analysis window:
• Check that samples that contain the same content are named with the same name.
In a Gene Study, the software assumes that wells with the same Target or Sample
name contain the same samples
• Adjust the baseline and threshold (C(t)) in the Quantitation tab to optimize the data in
each experiment before you add them to a Gene Study
• Select the well group you want to include in the Gene Study
The Study Setup tab (Figure 104) shows a list of all the experiments in the Gene Study.
• Add experiments. Click the Add Data Files button to select a file from a browser
window. To quickly add experiments to a Gene Study, drag the data files (.pcrd
extension) to the Gene Study window
TIP: In order to show data from one well group in the Gene Study, that group must
be selected before importing the Data file
• Remove experiments from this Gene Study. Select one or more files in the list and
click Remove
• Add notes about the Gene Study. Type in the Notes box to add comments about the
files and analysis in this Gene Study
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