CFX96 and CFX384 Systems Manual
103
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Figure 100. Layout of the Gene Expression tab in the Data Analysis window.
TIP: Right-click on the chart to select right-click menu options. Select Sort from
this menu to rearrange the order of the Target and Sample names in the chart.
Normalized Gene Expression
To normalize data use the measured expression level of one or more reference genes (targets)
as a normalization factor. Reference genes are targets that are not regulated in the biological
system being studied, such as actin, GAPDH, or Histone H3.
To set up normalized gene expression (ΔΔC(t)) analysis, follow these steps:
1. Open a data file (.pcrd extension).
2. Review the data in the Quantitation tab of the Data Analysis window. Make adjustments
to the data, such as changing the threshold and the Analysis Mode.
3. Click the Gene Expression tab.
4. Choose a control in the Samples tab of the Experiment Settings window. If a control is
assigned, the software normalizes the relative quantities for all genes to the control
quantity, which is set to 1.
5. Select reference genes for this experiment in the Target tab of the Experiment Settings
window. Gene expression analysis requires one reference among the targets in your
samples.
6. Select Normalized Expression (ΔΔC(t)) if it is not already selected, and then view the
expression levels in the Gene Expression tab.
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