BIO RAD CFX96 - Page 153

Index
144
Menu bar
Data analysis
, 71
Main software, 11, 12
Plate Editor, 43
Protocol Editor, 32
Mode
Analysis
, 75
Baseline Subtracted, 75
Baseline Subtracted Curve Fit, 75
Not Baseline Subtracted, 75
Temperature control, 37
Multiple instruments
Viewing
, 18
M-value, 105
N
Normalization factor
Relative quantity
, 117
Normalized expression
Formula
, 117
Formula for GOI, 119
Scaled to highest, 118
Scaled to lowest, 119
Standard deviation (SD), 119
Standard deviation formula, 118
Normalized GOI formula, 119
Normalizing
Gene expression
, 103
Not Baseline Subtracted mode, 75
Notes
Well
, 46
O
Open Lid button, 26
Opening
Lid
, 26
Melt Curve tab, 87
Protocol AutoWriter, 37
Protocol Editor, 31
Operation requirements, 1
Overview of CFX96 system, 2
P
Pause
Button
, 27
Run, 27
PCR
Supermixes
, 138
Plastic Consumables, 9
Plate
Contents of wells
, 46
Files, 20
Melt Curve Data, 90
Size, in Plate Editor, 43
Spreadsheet, 51, 90
Spreadsheet in Plate Editor, 42
Type, 43
Well contents, 46
Plate Editor
Clear Replicate #
, 48
Clear Wells button, 48
Clearing wells, 78
Concentration, 47
Dilution Series, 48
Excluding wells, 76
Experiment Settings, 48
Export plate spreadsheet, 52
Import plate spreadsheet, 52
Layout, 42
Magnification, 44
Menu bar, 43
Plate size, 43
Plate Type, 43
Replicate #, 47
Replicate Group, 47
Replicate series, 47
Sample Name, 47
Sample Type, 46
Scan Mode, 44, 45
Scientific notation, 43
Selecting fluorophores, 45
Spreadsheet, 43, 51
Target Name, 46
Toolbar, 42, 43
Units, 43
Well Groups Manager, 44
Well Notes, 48
Plate Editor window, 42
Plate Loading Guide, 42
Power
Input
, 3
Switch, 3
Protocol
Block mode
, 37
Calculated mode, 37
End point, 22
Express load, 22, 23
Files, 20
Steps, temperature, 31
Protocol AutoWriter, 39
Amplicon length, 38, 39