BIO RAD CFX96 - Page 154

CFX96 and CFX384 Systems Manual
145
Annealing temperature
, 38, 39
Button, 37
Creating a protocol, 39
Examples of protocol, 39
Run time, 38
Protocol Editor
Buttons
, 32, 33
Delete step, 37
Inserting GOTO, 35
Inserting gradient, 34
Inserting melt curve, 35
Menu bar, 32
Toolbar, 32
Window, 31
Q
QC Tab, 95
Quantitation
Description
, 72
Tab, 72
Tab layout, 72, 82
Quick guides, 11
R
Ramp rate, 37
Reaction efficiency (E)
Formula
, 84
Targets, 84
Reaction modules
Catalog numbers
, 138
Real-Time Status, 27
Regression Mode, 73
Regulatory compliance, iv
Relative quantity
Control sample selected
, 116
Described, 104
Formula, 116
GOI, 116
Normalization factor, 117
Normalized expression, 118
Standard deviation formula, 116
Replace Plate File, 28
Replicate #, 47
Replicate Group, 47
Replicate Series, 47
Report
Data file
, 97
Gene Study, 113
Requirements
Operation
, 1
Resume button, 27
Resuming a run, 27
Reverse transcription
Reagents
, 138
RFU
Allelic Discrimination
, 93, 96
End Point tab, 91, 92
Melt Curve tab, 87
Right-click
Gene expression graph
, 105
Selected Block(s) list, 25
RT_Data Folder, 61
Run
Adding repeats
, 27
Cancelling, 27
Flash block, 27
Pausing, 27
Skipping a step, 27
Stopping, 27
Run Details
Real-Time Status tab
, 27
Run Status, 26
Time Status tab, 28
Window, 25
Run Information
Tab
, 96
Run Status
Tab
, 26
Run time, 38
S
Safe use specifications, iv
Safety
Instruments
, iii
Warning labels, iii
Warnings, ii, iii
Sample
Name
, 46, 47
Type, 46
Sample Name Grouping, 50, 107
Sample Volume
Changing in end point
, 23
Sample volume
Changing
, 33
Scaling option in gene expression, 104
Scan Mode
All Channels
, 45, 60
FRET, 45, 60
Plate Editor, 44, 45
SYBR/FAM only, 45, 60
Scientific notation, 43
Select Fluorophores