BIO RAD CFX96 User Manual

Data Analysis Windows
92
The End Point tab shows the average RFU values to determine whether or not the target was
amplified by the last (end) cycle. Use these data to determine if a specific target sequence is
present (positive) in a sample. Positive targets have higher RFU values than the cutoff level you
define.
TIP: To create an end point protocol, open the Protocol tab (Experiment Setup
window) and select Options > End Point Only Run.
The software displays these data in the End Point tab:
• Settings. Adjust data analysis settings
• Results. Shows the results immediately after you adjust the Settings
• Well Selector. Select the wells with the end point data you want to show
• Well spreadsheet. Shows a spreadsheet of the end RFU collected in the selected wells
Figure 93. Layout of the End Point analysis tab.
The Results list includes this information:
•Lowest RFU value. Lowest RFU value in the data
• Highest RFU value. Highest RFU value in the data
• Negative Control Average. Average RFU for the wells that contain negative controls
• Cut Off Value. Calculated by adding the tolerance (RFU or Percentage of Range listed in
the Settings) and the average of the negative controls. Samples with RFUs that are
greater than the cut off value will be called “Positive”. To adjust the cut off value, change
the RFU or Percentage of Range
The Cut Off Value is calculated using this formula:
Select a tolerance by one of these methods:
• RFUs (default). Select this method to use an absolute RFU value for the tolerance. The
minimum RFU tolerance value is 2. The maximum is the absolute value of the highest
RFU value minus the absolute value of the lowest RFU value. The default RFU tolerance
value is 10% of the total RFU range
• Percent of Range. Select this method to use a percentage of the RFU range for the
tolerance. The minimum percent of range is 1 percent. The maximum percent of range is
99 percent. The default percent of range is 10 percent
Cut Off Value Negative Control Average Tolerance+=

BIO RAD CFX96 Specifications

General

Temperature Uniformity	±0.4 °C
Software	CFX Maestro Software
Detection Method	Real-time PCR (qPCR)
Sample Capacity	96-well plate
Excitation Source	LED
Ramp Rate	Up to 5°C/sec
Heating Rate	5°C/sec
Sensitivity	Single copy detection
Power Requirements	100-240 V, 50/60 Hz
Temperature Range	0–100 °C