BIO RAD CFX96 User Manual

Protocols
36
Figure 36 shows a melt curve step added after step 6:
Figure 36. Protocol with inserted melt curve step.
Step Options
To change a step option for the selected step:
1. Select a step by clicking on the step in the graphic or text view.
2. Click the Step Options button to open the Step Options window.
3. Add or remove options by entering a number, editing a number, or clicking a check box.
TIP: To hold a step forever (an infinite hold), enter zero (0.00) for the time.
Figure 37 shows the selected step with a gradient of 10
o
C. Notice that some options are not
available in a gradient step. A gradient step cannot include an increment or ramp rate change.
Figure 37. Step option for a gradient.
NOTE: A gradient runs with the lowest temperature in the front of the block (row H)
and the highest temperature in the back of the block (row A).
The Step Options window lists the following options you can add or remove from steps:
• Plate Read. Check the box to include a plate read
• Temperature. Enter a target temperature for the selected step
• Gradient. Enter a gradient range for the step

BIO RAD CFX96 Specifications

General

Temperature Uniformity	±0.4 °C
Software	CFX Maestro Software
Detection Method	Real-time PCR (qPCR)
Sample Capacity	96-well plate
Excitation Source	LED
Ramp Rate	Up to 5°C/sec
Heating Rate	5°C/sec
Sensitivity	Single copy detection
Power Requirements	100-240 V, 50/60 Hz
Temperature Range	0–100 °C