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biochrom ultrospec 7000 User Manual

biochrom ultrospec 7000
91 pages
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Ultrospec
7000/8000/9000
UV-Visible Spectrophotometers
User Manual
Biochrom US Telephone: 1-508-893-8999
84 October Hill Rd Toll Free: 1-800-272-2775
Holliston, MA Fax: 1-508-429-5732
01746-1388 support@hbiosci.com
USA www.biochromspectros.com

Table of Contents

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biochrom ultrospec 7000 Specifications

General IconGeneral
CategoryUV/Visible Spectrophotometer
Wavelength Range190 - 1100 nm
Optical SystemDouble beam
Wavelength Accuracy±0.5 nm
Stray Light<0.05%T at 220 nm and 340 nm
DetectorSilicon photodiode
ConnectivityUSB, RS-232
Power Requirements100-240 V, 50/60 Hz
Sample Holder10 mm cuvette
DisplayLCD
Data OutputRS-232, USB

Summary

Health and Safety

Safety

General safety warnings and potential hazards associated with the instrument's operation.

Unpacking and Installation

Instructions for safe unpacking and proper installation of the instrument.

Instrument Connections

Details on connecting the instrument to power and other required devices.

Equipment Operation

General guidance on the operation of the spectrophotometer.

Controls and Indicators

Identifies and explains the instrument's controls and indicator lights.

Instrument Preparation

Steps required to prepare the instrument before commencing measurements.

Post Run Procedures

Steps to follow after completing a measurement run.

Performance Validation

Procedures for checking and validating the instrument's performance.

User Maintenance

Information on maintenance tasks that can be performed by the user.

Troubleshooting

Common problems and their possible causes and solutions.

Customer Support Contacts

Contact information for customer support and technical assistance.

Service, Repair or Return

Guidelines for servicing, repairing, or returning the instrument.

Disposal

Instructions for the safe disposal of the instrument and accessories.

Introduction to the Ultrospec Spectrophotometer

Use with Datrys PC Software

Frequently Used Icons

Performing a Measurement

Ultrospec 7000/8000/9000 Measurement

Basic procedure for performing measurements with the instrument.

Lamp Mode

Types of Boxes

Settings

Date & Time Settings

How to set the instrument's date and time.

Regional Settings

Changing regional settings like language and number format.

Data Output Settings

Configuration of default saving and printing settings.

User Interface Settings

Settings for screen brightness, text entry, and screensaver.

Accessory Settings

Displays and allows setting parameters for fitted accessories.

Instrument Settings

General instrument configuration options.

Instrument Status

Displays the current operational status of the instrument.

Instrument Information

Displays serial number, UI version, and build dates.

Baseline and Lamp Settings

Options for managing baselines and lamp status/life.

User Access Control

Adding a User

Procedure for adding new users and assigning access levels.

Editing a User

How to modify existing user details and access rights.

Deleting a User

Steps for removing users from the instrument.

Editing User Access

Managing user login and access control settings.

Applications

Single Wavelength Application

Performing basic absorbance/transmission measurements.

Concentration via Factor

Measuring concentration using a predefined factor.

Wavescan

Performing wavelength scans and data manipulation.

Kinetics Measurements

Measuring absorbance changes over time.

Serial Kinetics Measurements

Measuring a single sample over time at intervals.

Parallel Kinetics Measurements

Measuring multiple samples over time concurrently.

Trace Manager for Data Manipulation

Overlays and manipulates scan and kinetic data files.

Standard Curve Creation

Creating calibration curves from standards.

Equation Editor for Custom Methods

Creating custom methods with calculations and thresholds.

Life Science Applications

Nucleic Acid Applications

Detailed applications for DNA, RNA, and Oligonucleotides.

CyDye DNA Measurements

Measuring labeling efficiency of fluorescent DNA probes.

Tm Calculation

Calculating theoretical melting point from DNA base sequence.

Protein Applications Overview

Methods for colorimetric protein assays and UV measurements.

BCA, Bradford, Lowry & Biuret Protein Assays

Outlines common protein assay methods.

BCA Protein Assay

Step-by-step guide for the Bicinchoninic Acid (BCA) protein assay.

Direct UV Protein Determination

Protein concentration via direct UV absorbance.

Protein UV Concentration

Using UV absorbance for protein concentration.

Protein A280 Measurement

Protein concentration measurement using A280 absorbance.

Saving and Printing

Saving Sample Data

Options for saving sample data (Internal, USB, CSV).

Automatic Saving

Configuring automatic saving of sample data.

Manual Saving

Procedures for manually saving sample data.

Exporting Data

Recalling and exporting data in different formats.

Sample Manager

Deleting Data From Internal Memory

How to delete files from the instrument's internal memory.

Accessing Sample Manager From Main Screen

Accessing Sample Manager from the main menu.

Accessing Sample Manager From Application

Accessing Sample Manager from within an application.

Recalled Files

How to view and use previously saved data files.

Saving Methods

Methods Saved To Internal Memory

Storing methods in the instrument's internal memory.

Methods Folder Structure

Structure and organization of method folders.

Renaming Method Folders

How to rename method folders.

Locking Saved Methods

Protecting methods from deletion with pass codes.

Deleting Saved Methods

Procedures for deleting saved methods.

Backing Up Method Folders To USB

Copying method folders to a USB stick.

Favourites Folder

Storing user-defined methods in a favorites folder.

Saving Methods To USB

Saving methods to a USB memory stick.

Printing

Internal Printer

Information on fitting and using the internal printer.

Print Via Computer (PVC)

Using PVC software to print data via a computer.

Bluetooth Printing

Information on the Bluetooth printing accessory.

Automatic Printing

Setting up automatic printing of sample data.

Manual Printing

Procedures for manually printing sample data.

Cell Changer

Cell Changer Operation

Setting up and operating the cell changer accessory.

Saving Methods & Results with Cell Changer

How methods with cell changer are saved.

Incorrect Cell Changer Fitting

Troubleshooting incorrect cell changer fitting.

Creating Standard Curve with Cell Changer

Using cell changer to create standard curves.

Sipper Accessory

Setting Sipper Defaults

Customizing default parameters for the Sipper accessory.

Sipper Calibration

Routine for calibrating the Sipper volume.

Sipper Wash Mode

Enabling the Sipper wash function.

Sipper Cleaning and Troubleshooting

Routine maintenance and troubleshooting for the Sipper.

Technical Specifications

Changing Tungsten and Deuterium Lamps

Table of Icons

Glossary of Boxes

Accessories

Installation

General instructions for installing accessories.

Passive Accessories

Accessories that do not interact with instrument software.

Single Cell Holder

Standard holder for 10mm pathlength cuvettes.

Variable Pathlength Cell Holder

Holder for cells with pathlengths 1-100mm.

Water Thermostatted Cell Holder

Holder for temperature-controlled measurements.

Film Holder

Holder for solid materials like films and lenses.

Test Tube Holder

Holder for measuring samples in test tubes.

Peltier Controlled Fluid Circulation

Accessory for temperature control of samples.

Peltier Controller General Information

Warnings and safety for the Peltier controller.

Peltier Controller Instrument Description

Overview of the Peltier Controller's features.

Peltier Controller Installation

Steps for installing the Peltier Controller.

Peltier Controller Operating Instructions

Manual operation details for the controller.

Peltier Controller Maintenance

Routine maintenance for the controller.

Peltier Controller Troubleshooting

Troubleshooting controller alarms and issues.

Active Accessories

Accessories that interact with instrument software.

8-Position Cell Changer

Accessory for automated sample measurement.

5-Position Thermostatted Cell Changer

Accessory for temp-controlled sample measurement.

Sipper & Flowcell

Accessory for automated sample handling via pump.

Sipper Troubleshooting

Common problems and solutions for the Sipper system.

Installing the Internal Printer

Step-by-step guide for installing the internal printer.

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