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biochrom ultrospec 8000 User Manual

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Ultrospec
7000/8000/9000
UV-Visible Spectrophotometers
User Manual
Biochrom US Telephone: 1-508-893-8999
84 October Hill Rd Toll Free: 1-800-272-2775
Holliston, MA Fax: 1-508-429-5732
01746-1388 support@hbiosci.com
USA www.biochromspectros.com

Table of Contents

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biochrom ultrospec 8000 Specifications

General IconGeneral
Brandbiochrom
Modelultrospec 8000
CategoryMedical Equipment
LanguageEnglish

Summary

1. Health & Safety

1.1 Safety Information

General hazards, warning labels, and safe operating practices for the instrument.

1. Health & Safety (continued)

1.2 Unpacking and Installation

Guidance on safe unpacking, installation site, and environmental conditions for the instrument.

1.2.1 Instrument Connections

Details on connecting power, PC, and accessories via ports and plugs.

1.3 Equipment Operation

1.3.3 Instrument Preparation

Steps for preparing the instrument, including warm-up, connections, and cuvette selection.

1.4 User Maintenance

1.4 User Maintenance (continued)

1.4.1 Troubleshooting

Lists common problems, possible causes, and solutions for instrument operation.

5. Performing a Measurement

5.1 Ultrospec 7000/8000/9000 Measurement Methods

Explains dual beam operation and measurement methods against air, solvent, or matched cuvettes.

8. Settings

8. Settings (continued)

9. User Access

User Account Management

Managing user accounts, access rights, and privileges on the instrument.

10. Applications

10.1 Single Wavelength

Performing absorbance/transmission measurements at a single wavelength.

10. Applications (continued)

10.2 Concentration via Factor

Measuring concentration by multiplying absorbance by a predefined or calculated factor.

10.3 Wavescan

Measuring absorbance/transmission across a wavelength range with data manipulation options.

10.4 Kinetics

Measuring absorbance change at a single wavelength over time to determine reaction rates.

10.4.1 Serial Kinetics Measurements

Measuring absorbance of a single sample over time at specified intervals.

10.4.2 Parallel Kinetics Measurements

Measuring absorbance of multiple samples simultaneously over time at specified intervals.

10.5. Trace Manager – Overlaying & Manipulating Files

10.6. Standard Curve

10.7. Equation Editor

11. Life Science Applications

11. Life Science Applications (continued)

11.1.1 DNA, RNA & Oligo Analysis

Details on quantifying DNA, RNA, and Oligos, and purity checks.

11.2. Protein Applications

11.2.2 BCA Protein Assay

Detailed explanation of the BCA assay principle and procedure.

11.2. Protein Applications (continued)

11.2.3 Direct UV Protein Determination

Direct UV method for protein determination using absorbance at 280 nm.

11.2.5 Protein A280 Analysis

Protein determination using absorbance at 280 nm with various calculation options.

12. Saving & Printing Sample Data

12. Saving & Printing (continued)

13. Sample Manager

13. Sample Manager (continued)

14. Saving Methods

14. Saving Methods (continued)

15. Printing

16. Cell Changer

16. Cell Changer (continued)

17. Sipper

17. Sipper (continued)

19. Changing Tungsten and Deuterium Lamps

19. Changing Lamps (continued)

Lamp Removal/Replacement & Resetting Lamp Life

Procedures for replacing lamps and resetting lamp life in the software.

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