29004782UM AA 10/2011 3
1. HEALTH & SAFETY 4
1.1. General Safety 4
1.1.1. General Hazards 4
1.2. Unpacking & Installation 5
1.2.1. Instrument Connections 6
1.3. Equipment Operation 7
1.3.1. Controls and Indicators 7
1.3.2. Intended Use 7
1.3.3. Instrument Preparation 7
1.3.4. Post Run Procedures 8
1.3.5. Performance Validation 8
1.4. User Maintenance 8
1.4.1. Troubleshooting 9
1.5. Customer Support Contacts 10
1.6. Service, Repair or Return 10
1.7. Disposal 10
2. INTRODUCTION TO THE ULTROSPEC 7000/8000/
9000 SPECTROPHOTOMETER 11
3. USE WITH Datrys PC SOFTWARE 12
4. FREQUENTLY USED ICONS 13
5. PERFORMING A MEASUREMENT 14
5.1. Ultrospec 7000/8000/9000 14
6. LAMP MODE 15
7. TYPES OF BOXES 16
8. SETTINGS 17
8.1. Date and time 17
8.2. Regional 17
8.3. Data Output 18
8.4. User Interface 18
8.5. Accessories 18
8.6. Instrument Settings 18
8.7. Instrument Status 18
8.8. Instrument Information 19
8.9. Instrument Settings 19
8.10. Lamp Settings 19
9. USER ACCESS 20
9.1. Adding a user 20
9.2. Editing a user 20
9.3. Deleting a user 20
9.4. Editing user access 21
10. APPLICATIONS 22
10.1. Single Wavelength 22
10.2. Concentration via factor 23
10.3. Wavescan 24
10.4. Kinetics 25
10.4.1. Serial kinetics measurements 26
10.4.2. Parallel kinetics measurements 28
10.5. Trace Manager – Overlaying & manipulating
wavescan and kinetics files 30
10.6. Standard Curve 32
10.7. Equation Editor 34
11. LIFE SCIENCE APPLICATIONS 40
11.1. Nucleic Acid Applications 41
11.1.1. DNA, RNA & Oligo 41
11.1.2. CyDye™ DNA 43
11.1.3. T
m
Calculation 44
11.2. Protein Applications 47
11.2.1. BCA, Bradford, Lowry & Biuret Protein
Assays 47
11.2.2. Determination of Protein Concentration
using the BCA protein assay 47
11.2.3. Determination of Protein Concentration
using direct UV methods 50
11.2.4. Protein UV 51
11.2.5. Protein A280 52
12. SAVING AND PRINTING 53
12.1. Saving Sample Data 53
12.1.1. Internal 53
12.1.2. USB 53
12.1.3. USB csv 53
12.2. Automatic Saving 54
12.3. Manual Saving 54
12.4. Exporting Data 54
13. SAMPLE MANAGER 55
13.1. Deleting data from the internal memory 55
13.2. Accessing Sample Manager from the main
screen 56
13.3. Accessing Sample Manager from within an
application 56
13.4. Recalled files 57
14. SAVING METHODS 58
14.1. Methods saved to the internal memory 58
14.2. Methods folder 58
14.3. Renaming methods folder 59
14.4. Locking saved methods 59
14.5. Deleting saved methods 59
14.6. Backing up method folders to USB 60
14.7. Favourites folder 60
14.8. Saving methods to USB 60
15. PRINTING 61
15.1. Internal printer 61
15.2. Print via computer (PVC) 61
15.3. Bluetooth 61
15.4. Automatic printing 61
15.5. Manual printing 61
ACCESSORIES 62
Fitting 62
Using the Cell Charger to Create a
Standard Curve
Cleaning/Maintenance/Troubleshooting
CHANGING TUNGSTEN AND DEUTERIUM LAMPS
29004782UM AA 10/2011 3
1. HEALTH & SAFETY 4
1.1. General Safety 4
1.1.1. General Hazards 4
1.2. Unpacking & Installation 5
1.2.1. Instrument Connections 6
1.3. Equipment Operation 7
1.3.1. Controls and Indicators 7
1.3.2. Intended Use 7
1.3.3. Instrument Preparation 7
1.3.4. Post Run Procedures 8
1.3.5. Performance Validation 8
1.4. User Maintenance 8
1.4.1. Troubleshooting 9
1.5. Customer Support Contacts 10
1.6. Service, Repair or Return 10
1.7. Disposal 10
2. INTRODUCTION TO THE ULTROSPEC 7000/8000/
9000 SPECTROPHOTOMETER 11
3. USE WITH Datrys PC SOFTWARE 12
4. FREQUENTLY USED ICONS 13
5. PERFORMING A MEASUREMENT 14
5.1. Ultrospec 7000/8000/9000 14
6. LAMP MODE 15
7. TYPES OF BOXES 16
8. SETTINGS 17
8.1. Date and time 17
8.2. Regional 17
8.3. Data Output 18
8.4. User Interface 18
8.5. Accessories 18
8.6. Instrument Settings 18
8.7. Instrument Status 18
8.8. Instrument Information 19
8.9. Instrument Settings 19
8.10. Lamp Settings 19
9. USER ACCESS 20
9.1. Adding a user 20
9.2. Editing a user 20
9.3. Deleting a user 20
9.4. Editing user access 21
10. APPLICATIONS 22
10.1. Single Wavelength 22
10.2. Concentration via factor 23
10.3. Wavescan 24
10.4. Kinetics 25
10.4.1. Serial kinetics measurements 26
10.4.2. Parallel kinetics measurements 28
10.5. Trace Manager – Overlaying & manipulating
wavescan and kinetics files 30
10.6. Standard Curve 32
10.7. Equation Editor 34
11. LIFE SCIENCE APPLICATIONS 40
11.1. Nucleic Acid Applications 41
11.1.1. DNA, RNA & Oligo 41
11.1.2. CyDye™ DNA 43
11.1.3. T
m
Calculation 44
11.2. Protein Applications 47
11.2.1. BCA, Bradford, Lowry & Biuret Protein
Assays 47
11.2.2. Determination of Protein Concentration
using the BCA protein assay 47
11.2.3. Determination of Protein Concentration
using direct UV methods 50
11.2.4. Protein UV 51
11.2.5. Protein A280 52
12. SAVING AND PRINTING 53
12.1. Saving Sample Data 53
12.1.1. Internal 53
12.1.2. USB 53
12.1.3. USB csv 53
12.2. Automatic Saving 54
12.3. Manual Saving 54
12.4. Exporting Data 54
13. SAMPLE MANAGER 55
13.1. Deleting data from the internal memory 55
13.2. Accessing Sample Manager from the main
screen 56
13.3. Accessing Sample Manager from within an
application 56
13.4. Recalled files 57
14. SAVING METHODS 58
14.1. Methods saved to the internal memory 58
14.2. Methods folder 58
14.3. Renaming methods folder 59
14.4. Locking saved methods 59
14.5. Deleting saved methods 59
14.6. Backing up method folders to USB 60
14.7. Favourites folder 60
14.8. Saving methods to USB 60
15. PRINTING 61
15.1. Internal printer 61
15.2. Print via computer (PVC) 61
15.3. Bluetooth 61
15.4. Automatic printing 61
15.5. Manual printing 61
ACCESSORIES 62
Fitting 62
CELL CHARGER 63
Operation 63
Saving Method & Results 64
Incorrect Fitting 64
Using the Cell Charger to Create a
Standard Curve 64
Sipper 65
Setting Sipper defaults 65
Calibration 66
Wash Mode 66
Cleaning/Maintenance/Troubleshooting 67
TECHNICAL SPECIFICATIONS 68
CHANGING TUNGSTEN AND DEUTERIUM LAMPS 69
TABLE OF ICONS 71
GLOSSARY OF BOXES 75
LEGAL 79
CONTENTS:
1. HEALTH & SAFETY 4
1.1. General Safety 4
1.1.1. General Hazards 4
1.2. Unpacking & Installation 5
1.2.1. Instrument Connections 6
1.3. Equipment Operation 7
1.3.1. Controls and Indicators 7
1.3.2. Intended Use 7
1.3.3. Instrument Preparation 7
1.3.4. Post Run Procedures 8
1.3.5. Performance Validation 8
1.4. User Maintenance 8
1.4.1. Troubleshooting 9
1.5. Customer Support Contacts 10
1.6. Service, Repair or Return 10
1.7. Disposal 10
2. INTRODUCTION TO THE ULTROSPEC 7000/8000/
9000 SPECTROPHOTOMETER 11
3. USE WITH Datrys PC SOFTWARE 12
4. FREQUENTLY USED ICONS 13
5. PERFORMING A MEASUREMENT 14
5.1. Ultrospec 7000/8000/9000 14
6. LAMP MODE 15
7. TYPES OF BOXES 16
8. SETTINGS 17
8.1. Date and time 17
8.2. Regional 17
8.3. Data Output 18
8.4. User Interface 18
8.5. Accessories 18
8.6. Instrument Settings 18
8.7. Instrument Status 18
8.8. Instrument Information 19
8.9. Instrument Settings 19
8.10. Lamp Settings 19
9. USER ACCESS 20
9.1. Adding a user 20
9.2. Editing a user 20
9.3. Deleting a user 20
9.4. Editing user access 21
10. APPLICATIONS 22
10.1. Single Wavelength 22
10.2. Concentration via factor 23
10.3. Wavescan 24
10.4. Kinetics 25
10.4.1. Serial kinetics measurements 26
10.4.2. Parallel kinetics measurements 28
10.5. Trace Manager – Overlaying & manipulating
wavescan and kinetics files 30
10.6. Standard Curve 32
10.7. Equation Editor 34
11. LIFE SCIENCE APPLICATIONS 40
11.1. Nucleic Acid Applications 41
11.1.1. DNA, RNA & Oligo 41
11.1.2. CyDye™ DNA 43
11.1.3. T
m
Calculation 44
11.2. Protein Applications 47
11.2.1. BCA,
Bradford, Lowry & Biuret Protein
Assays 47
11.2.2. Determination of Protein Concentration
using the BCA protein assay 47
11.2.3. Determination of Protein Concentration
using direct UV methods 50
11.2.4. Protein UV 51
11.2.5. Protein A280 52
12. SAVING AND PRINTING 53
12.1. Saving Sample Data 53
12.1.1. Internal 53
12.1.2. USB 53
12.1.3. USB csv 53
12.2. Automatic Saving 54
12.3. Manual Saving 54
12.4. Exporting Data 54
13. SAMPLE MANAGER 55
13.1. Deleting data from the internal memory 55
13.2. Accessing Sample Manager from the main
screen 56
13.3. Accessing Sample Manager from within an
application 56
13.4. Recalled files 57
14. SAVING METHODS 58
14.1. Methods saved to the internal memory 58
14.2. Methods folder 58
14.3. Renaming methods folder 59
14.4. Locking saved methods 59
14.5. Deleting saved methods 59
14.6. Backing up method folders to USB 60
14.7. Favourites folder 60
14.8. Saving methods to USB 60
15. PRINTING 61
15.1. Internal printer 61
15.2. Print via computer (PVC) 61
15.3. Bluetooth 61
15.4. Automatic printing 61
15.5. Manual printing 61
ACCESSORIES 62
Fitting 62
CELL CHARGER 63
Operation 63
Saving Method & Results 64
Incorrect Fitting 64
Using the Cell Charger to Create a
Standard Curve 64
Sipper 65
Setting Sipper defaults 65
Calibration 66
Wash Mode 66
Cleaning/Maintenance/Troubleshooting 67
TECHNICAL SPECIFICATIONS 68
CHANGING TUNGSTEN AND DEUTERIUM LAMPS 69
TABLE OF ICONS 71
GLOSSARY OF BOXES 75
LEGAL 79
29004782UM AA 10/2011 3
1. HEALTH & SAFETY 4
1.1. General Safety 4
1.1.1. General Hazards 4
1.2. Unpacking & Installation 5
1.2.1. Instrument Connections 6
1.3. Equipment Operation 7
1.3.1. Controls and Indicators 7
1.3.2. Intended Use 7
1.3.3. Instrument Preparation 7
1.3.4. Post Run Procedures 8
1.3.5. Performance Validation 8
1.4. User Maintenance 8
1.4.1. Troubleshooting 9
1.5. Customer Support Contacts 10
1.6. Service, Repair or Return 10
1.7. Disposal 10
2. INTRODUCTION TO THE ULTROSPEC 7000/8000/
9000 SPECTROPHOTOMETER 11
3. USE WITH Datrys PC SOFTWARE 12
4. FREQUENTLY USED ICONS 13
5. PERFORMING A MEASUREMENT 14
5.1. Ultrospec 7000/8000/9000 14
6. LAMP MODE 15
7. TYPES OF BOXES 16
8. SETTINGS 17
8.1. Date and time 17
8.2. Regional 17
8.3. Data Output 18
8.4. User Interface 18
8.5. Accessories 18
8.6. Instrument Settings 18
8.7. Instrument Status 18
8.8. Instrument Information 19
8.9. Instrument Settings 19
8.10. Lamp Settings 19
9. USER ACCESS 20
9.1. Adding a user 20
9.2. Editing a user 20
9.3. Deleting a user 20
9.4. Editing user access 21
10. APPLICATIONS 22
10.1. Single Wavelength 22
10.2. Concentration via factor 23
10.3. Wavescan 24
10.4. Kinetics 25
10.4.1. Serial kinetics measurements 26
10.4.2. Parallel kinetics measurements 28
10.5. Trace Manager – Overlaying & manipulating
wavescan and kinetics files 30
10.6. Standard Curve 32
10.7. Equation Editor 34
11. LIFE SCIENCE APPLICATIONS 40
11.1. Nucleic Acid Applications 41
11.1.1. DNA, RNA & Oligo 41
11.1.2. CyDye™ DNA 43
11.1.3. T
m
Calculation 44
11.2. Protein Applications 47
11.2.1. BCA, Bradford, Lowry & Biuret Protein
Assays
using the BCA protein assay
13.1. Deleting data from the internal memory
13.2. Accessing Sample Manager from the main
screen
13.3. Accessing Sample Manager from within an
application
14.1. Methods saved to the internal memory
14.3. Renaming methods folder
14.4. Locking saved methods
14.5. Deleting saved methods
14.6. Backing up method folders to USB
14.8. Saving methods to USB
15.2. Print via computer (PVC)
61
ACCESSORIES 62
Fitting 62
CELL CHARGER 63
Operation 63
Saving Method & Results 64
Incorrect Fitting 64
Using the Cell Charger to Create a
Standard Curve 64
Sipper 65
Setting Sipper defaults 65
Calibration 66
Wash Mode 66
Cleaning/Maintenance/Troubleshooting 67
TECHNICAL SPECIFICATIONS 68
CHANGING TUNGSTEN AND DEUTERIUM LAMPS 69
TABLE OF ICONS 71
GLOSSARY OF BOXES 75
LEGAL 79
CONTENTS:
16.
17.
18.
19.
20.
21.
16.1.
16.2.
16.3.
16.4.
17.1.
17.2.
17.3.
17.4.
61
61
62
62
62
63
63
64
64
65
66
67
69
73
20
20
20
20
20
21
21
22
23
24
25
27
29
31
33
39
40
40
42
43
46
46
46
49
50
51
52
52
52
52
52
53
53
53
54
54
55
55
56
57
57
57
58
58
58
59
59
59
60
60
60
60
60
60
1.1. Safety
To Next Page
Loading...
