Biocontrol LIGHTNING MVP ICON User Manual

SurfaceSamplingDevice
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GENERALDESCRIPTION
TheLIGHTNINGMVPICONSurfaceSamplingDeviceisaself‐
containeddeviceforusewiththeLIGHT NINGMVPICON™or
LIGHTNING MVP
®
systemsforqualityandHACCP‐related
monitoringoftheenvironment.TheMVPICONand
LIGHTNINGMVPsystemsdetectfoodandmicrobiological
residuesbymeasuringAdenosinetriphosphate(ATP)using
luciferasebioluminescence.ATPisfoundinmostfood
residuesandallbacteria,yeastandmoldcells.
PartNo.64003‐100(100tests)
KITCOMPONENTS
Oneboxof100surfacesamplingdevices
IMPORTANTUSAGEGUIDELINES
Itiscriticaltoinsertthesamplingdevice
intotheMVPICONwiththetwoflat
sidesofthesamplingdevice’sbase
chamberfacingleftandright.Improper
samplingdeviceorientationmayresultin
damagetotheinstrument.
Donotinsertwetorcontaminated
samplingdevicesintotheinstrument.Wipecondensate
from
exteriorofdevicebeforeusewithaclean,lint‐freecloth.
Avoidhandlingthedevicewithwetorcontaminatedhands.
Alwaysremovesamplingdevicesfrominstrumentafter
reading.
DonotstoretheMVPICONorMVPinstrumentonitssideor
back.Avoidstoringinhumidareaswherewater
leaksare
possible.
TESTPROCEDURE
A.CollectingSample
(a) Twisttoremovesamplingdevicefrombodytube.The
swabtipmustbemoist.Iffullysaturated,anyexcess
wettingagentshouldbeblottedoffbygentlypressing
theswabtipagainsttheinteriorofthebodytube.Be
carefulnottotouchtheswaborinsidethesampling
device
withfingers.
(b) Thoroughlyswabastandard10x10cm(4x4in)areaof
interestforatypicalflatsurface.Forirregularlyshaped
surfaces,ensureswabbingtechniqueremainsconsistent
eachtimeyouswab.Reinserttheswabintothebody
tubeusingatwistingmotiontoensureit
issealed.
Note:Afterswabbing,thesamplingdeviceisstableforone
hourpriortoactivation.
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B.ActivatingDeviceandReadingResults

(a) Toactivatethesamplingdevice,holduprightandpush
theplungerdownuntilitisfullydepressed.Thereagents
willbedispensedthroughthesamplingdevicewashing
thesampleofftheswabtipandintothecollectiontube.
(b) InsertthesamplingdeviceintotheMVPICONorMVP
andreadtheresults.WheninsertingintotheMVPICON,
ensure
theflatsidesofthesamplingdevicebase
chamberfaceleftandrightintheinstrument.
Note:Resultsshouldbereadwithintwominutesof
activation.PleaserefertotheMVPICONUserGuideorMVP
SystemGuideforoperatinginstructions.
INTERPRETINGRESULTS
(a) WhenusingdefaultthresholdsintheMVPICONorMVP
systems,readingsof2.5Zonesorlessindicatethe
surfaceisconsideredclean.
(b) Readingsbetween2.6‐3.0Zonesindicateawarningthe
surfaceisnotadequatelyclean.
(c) Ifthereadingisgreaterthan3.0Zones,thesurfaceis

considereddirtyandshouldbere‐cleanedandre‐tested.
CONTROLS
A. PositiveControl
ItisrecommendedLIGHTNINGMVPICONATPPositive
Controls(PartNo.64001‐25)beruneveryotherdaytoverify
integrityofthereagentsystem.
(a) Apositivecontrolshouldyieldaresultfrom3.1to4.3
Zones.SeeATPPositiveControlDirectionsforUsefor
additionalinformation.
B. NegativeControl
AnegativecontrolisanunusedyetactivatedLIGHTNINGMVP
ICONSurfaceSamplingDevice.
(a) Negativecontrolresultsshouldreadbetween1.0to2.5
Zones.
(b) Readingsabove2.5mayindicatecontaminationofthe
MVPICONorMVPsamplingchamberorsamplingdevice.
SeeATPPositiveControlDirectionsforUsefor
additional
information.ContactBioControlTechnicalSupportat
800.245.0113formoreinformation.
PRECAUTIONS
Theappearanceofayellowbandontheswabtipisnormal
andwillnotaffectperformance.
Ifaccidentalactivationpriortosamplingoccurs,donotuse
device.
FlatSide

Biocontrol LIGHTNING MVP ICON Specifications

General

Brand	Biocontrol
Model	LIGHTNING MVP ICON
Category	Laboratory Equipment
Language	English

Summary

Product Overview and Usage Guidelines

General Description

Self-contained device for quality monitoring, detecting food and microbiological residues using ATP.

Kit Components

Contains one box of 100 surface sampling devices for use with MVP ICON/MVP systems.

Important Usage Guidelines

Proper device insertion, handling, and storage are critical to prevent instrument damage and ensure accurate results.

Test Procedure

Collecting Sample

Swab a 10x10 cm area, ensuring the tip is moist. Reinsert swab into the body tube.

Activating and Reading Results

Push plunger to dispense reagents, then insert device into MVP ICON for reading within two minutes.

Interpreting Results

Clean Surface Thresholds

Readings of 2.5 Zones or less indicate a clean surface based on default thresholds.

Warning Thresholds

Readings between 2.6 - 3.0 Zones suggest the surface is not adequately clean.

Dirty Surface Thresholds

Readings greater than 3.0 Zones indicate a dirty surface requiring re-cleaning and re-testing.

Controls and Precautions

Positive Control Verification

Run ATP Positive Controls periodically. Expected result: 3.1-4.3 Zones.

Negative Control Verification

Use unused activated device. Expected result: 1.0-2.5 Zones. Above 2.5 may indicate contamination.

General Precautions

Yellow band on swab is normal. Do not use if accidentally activated before sampling.

Storage and Warranty Information

Storage Instructions

Store devices between 2°C-30°C (36°F–86°F), protect from sunlight, and reseal unused bags.

Product Warranty

Warrants product free from defects in materials/workmanship when stored/used as intended.